期刊
ELECTROANALYSIS
卷 33, 期 11, 页码 2381-2391出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/elan.202100251
关键词
Cholesterol; Microbial Biosensor; Ionic Liquid; MWCNT
资金
- Ege University Research Fund [15 FEN 021]
In this study, an amperometric cholesterol biosensor based on cholesterol oxidase from Pseudomonas sp. and catalase was developed, immobilized in carbon paste electrode (CPE) modified with multiwall carbon nanotubes (MWCNT) and ionic liquid (IL). The biosensor exhibited linear relationship in the range of 5-600 mu M with a low detection limit of 1.52 mu M, showing good sensitivity and high selectivity for measuring cholesterol levels in lyophilized serum samples.
In this study we report the development of an amperometric cholesterol biosensor based on cholesterol oxidase from Pseudomonas sp. and catalase immobilized in carbon paste electrode (CPE) modified with multiwall carbon nanotubes (MWCNT) and ionic liquid (IL). The working electrode (CPE/MWCNT-IL/Microorganism (MO)-Catalase) was characterized by impedance spectroscopy and cyclic voltammetry at different stages of its construction. This proposed cholesterol biosensor performed linear relationship in the range of 5-600 mu M with a low detection limit of 1.52 mu M. The biosensor showed good sensitivity and high selectivity and it was successfully applied for the measurement of cholesterol levels in lyophilized serum samples.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据