期刊
DYES AND PIGMENTS
卷 197, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.dyepig.2021.109823
关键词
Bis-reaction-triggered; Cascade-responsive; Specific detection of Cys; Two-stage response; Dynamic cell-imaging
资金
- National Natural Science Foundation of China [21871122, 21431002]
- Fundamental Research Funds for the Central Universities [lzujbky-2018-kb12]
The newly constructed Cys fluorescent probe CT-C showed highly specific and two-stage fluorescence response towards Cys, overcoming interference from Hcy and GSH. This probe enables specific fluorescence visualization of Cys in cells.
The striking similarities in chemical structures and properties between cysteine (Cys), homocysteine (Hcy) and glutathione (GSH) bring huge interferences to achieve specific detection of Cys, limiting the further physiological and pathological researches on Cys. Herein, a bis-reaction-triggered cascade-responsive highly specific Cys fluorescent probe CT-C was constructed, containing coumarin group as fluorophore, alpha,beta-unsaturated ethanoyl as a Michael-addition site and 2-thiophenecarbonyl moiety as a nucleophilic substitution site, which overcame the interference of Hcy and GSH by two successive reaction processes. CT-C presented a two-stage fluorescence response towards Cys, a fast and strong turn-on response in the first process and a lengthy on-off response in the subsequent second process, while CT-C only displayed a very slow and weak fluorescence enhancement towards Hcy, and a slight fluorescence change towards GSH. To our knowledge, CT-C was the first reported probe utilizing two-stage cascade response for specific single detection of Cys. Furthermore, the novel two-stage response mode was also useful for the specific fluorescence visualization of exogenous Cys in BHK-21 cells.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据