期刊
DYES AND PIGMENTS
卷 191, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.dyepig.2021.109349
关键词
Esterase; Fluorescent probes; Benzothiazole-based derivative; Bioimaging; Excited state intramolecular proton transfer
资金
- National Natural Science Foundation of China [21978131, 21878156]
- Six Talent Peaks Project in Jiangsu Province [XCL034]
- National Key Research and Development Program of China [2018YFA0902200]
- Project of Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
This study designed and synthesized three fluorescent probes capable of efficiently detecting esterase, exhibiting high sensitivity and specificity in living cells. Test strip experiments also demonstrated their ability to recognize esterase with remarkable color changes.
Esterase plays a crucial role in regulating various biological activities in living organisms. Detecting esterase levels can be beneficial for the treatment of various diseases. In this study, we have designed and synthesized ESIPT (excited state intramolecular proton transfer)-inspired fluorescent probes HBT-EA, HBT-MA-EA, and HBTPy-EA, based on benzothiazole derivatives, to detect and identify esterase. All three probes exhibited remarkable enhancement of fluorescent signal in the presence of esterase. These probes were also utilized to detect esterase in living cells with high sensitivity and specificity. Furthermore, a test strips experiment suggests that the probes can recognize esterase accompanied by remarkable color change. Overall, our data suggest that these probes are effective tools for the detection of endogenous esterase, and we expect that they will be further applied in biological and materials science research.
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