期刊
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
卷 121, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.dci.2021.104107
关键词
IL-22; IL-22RA1; Mandarin fish; Expression
资金
- National Natural Science Foundation of China [31702369, 32002422]
- China Agriculture Research System [CARS-46]
- special top talent plan One Thing One Decision (Yishi Yiyi) in Shandong Province, China [(2018)27]
- First Class Fishery Discipline programme in Shandong Province, China [(2018)8]
The study revealed that mandarin fish IL-22 exhibits its bioactivity in a cell-specific manner in intestinal cells, as reflected in the restrictive expression of its receptor unit, IL-22RA1. Other tested cells did not respond to IL-22, indicating the cell-specificity of its effects.
IL-22, a multifunctional cytokine, acts as an important regulator in host immunity in mammals. IL-22 homologues have been characterized in several species of fish, with its expression found in multiple tissues/cells in fish, but its target cells have not been fully analyzed. In the present research, different organ/tissue isolated cells were examined for the expression of IL-22 and the induced IL-22 responses in mandarin fish. The mandarin fish IL-22 was found to be expressed in all these tested cells with high basal expression in intestinal cells. The HKLs showed low basal expression but significant increase in expression of IL-22 after LPS treatment or bacterial infection. Only intestinal cells showed response to IL-22 by enhanced expression of hepcidin, LEAP2 and IL-22BP, with unresponsiveness observed in other tested cells, which indicated the cell-specificity of IL-22 bioactivity in mandarin fish. One of the heterodimeric receptor components for IL-22, the IL-22RA1, was cloned in mandarin fish, with four tandem fibronectin type III (FNIII) domains identified in its extracellular part. IL-22RA1 exhibited an intestinal cell-specific expression pattern, although another receptor component of IL-22, IL-10R2, displayed constitutive expressions in all these tested cells. The present study reveals that the mandarin fish IL-22 exhibits its bioactivity in a cell-specific manner in intestinal cells, which is reflected in the restrictive expression of its receptor unit, IL-22RA1.
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