期刊
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
卷 121, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.dci.2021.104091
关键词
CIITA; Cynoglossus semilaevis; MHC II; Immunity; Vibrio harveyi
资金
- National Natural Science Foundation of China [31702373, 31802332]
- China Agriculture Research System [CARS47G03]
- Central Publicinterest Scientific Institute Basal Research Fund, CAFS [2020TD19]
The research found that Cs-CIITA plays a crucial role in the inflammatory response of C. semilaevis against V. harveyi, regulating the expression of other inflammation-related genes through MHC II transcriptional regulation.
In mammals, Class II, major histocompatibility complex (MHC II) transactivator (CIITA) recognizes microbial pathogens and triggers immune responses. In Chinese tongue sole Cynoglossus semilaevis, Cs-CIITA was prevalently expressed in various tissues. Cs-CIITA, Cs-MHC IIA and Cs-MHC IIB were expressed significantly higher in skin in susceptible families infected with Vibrio harveyi, while higher expression of Cs-CIITA and Cs-MHC IIB was examined in liver in resistant families. In addition, the three genes were up-regulated in gill, skin, intestine, liver, spleen and kidney at 48 h or 72 h after V. harveyi infection. Furthermore, the three genes were co-expressed in the epithelial mucous cells of gill, skin, and intestine. Knockdown of Cs-CIITA regulates the expression of other inflammation-related genes, including CD40, IL-1 beta, IL-8, RelB, NF kappa B, and Myd88. These results suggest that CIITA functions in the inflammatory responses of C. semilaevis against V. harveyi, via MHC II transcriptional regulation.
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