期刊
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
卷 122, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.dci.2021.104105
关键词
NLK; MAVS; Interferon; SVCV; Black carp
资金
- National Key Research and Development Program of China [2019YFD0900303]
- National Natural Science Foundation of China [31920103016]
- Hunan Provincial Science and Technology Department [2019RS1038, 2019RS2032, 2019RS5001]
- Training Program for Excellent Young Innovators of Changsha [kp2009046]
- Modern Agricultural Industry Program of Hunan Province, National Students' Platform for Innovation and Entrepreneurship Training Program [201910542044]
- Hunan province college students research learning and innovative experiment project [S202010542041, S202010542064]
- Degree & Postgraduate Education Reform Project of Hunan Province [2019JGYB095]
- Excellent Education Team for Postgraduate in Hunan Province
The study cloned and characterized the NLK homolog bcNLK in black carp, demonstrating its association with MAVS and inhibition of MAVS-mediated antiviral signaling.
Mammalian Nemo-like kinase (NLK) plays important roles in multiple biological processes including immune response; however, the roles of teleost NLK remain largely unknown. In the present study, the NLK homolog (bcNLK) of black carp (Mylopharyngodon piceus) has been cloned and characterized. The coding region of bcNLK consists of 1427 nucleotides and encodes 476 amino acid, including two low complexity region (LCR) domains at the N-terminus and a serine/threonine protein kinase catalytic (S-TKc) domain in the middle region. The transcription of bcNLK are promoted after spring viremia of carp virus (SVCV) infection and poly (I:C) stimulation in host cells, but not post LPS treatment. bcNLK exhibits weak impact on the transcription of interferon (IFN) promoter in the reporter assay, however, black carp MAVS (bcMAVS)-mediated IFN promoter transcription is remarkably dampened by bcNLK. The interaction between bcNLK and bcMAVS is detected through the coimmunoprecipitation assay. Accordingly, the plaque assay results show that bcMAVS-mediated antiviral ability is impaired by bcNLK. Moreover, knockdown of bcNLK in host cells leads to the enhanced antiviral ability against SVCV. All these data support the conclusion that black carp NLK associates with MAVS and inhibited MAVS-mediated antiviral signaling.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据