期刊
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
卷 119, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.dci.2021.104025
关键词
microRNA; IRAK1; Negative regulation; Innate immune
资金
- National Natural Science Foundation of China [31802325]
- National Key Research and Development Project [2018YFD0900301]
This study elucidated the mechanism by which miR-1388-5p regulates the NF-kappa B signaling pathway by targeting the IRAK1 gene, enriching the miRNA-mediated regulatory networks in fish innate immunity.
Innate immune response is an important response mechanism for the host to achieve self-protection, and it plays an important role in identifying pathogens and resisting pathogen invasion. Growing evidences have shown that microRNA functions as a crucial regulator involved in the host innate immune response. In this study, the regulations of miR-1388-5p to regulate NF-kappa B signaling pathways via targeting the IRAK1 gene was studied in miiuy croaker. First, through bioinformatics software prediction, we found that IRAK1 is the direct target of miR-1388-5p, and then the prediction results were verified by using dual-luciferase assays. Next, we found that both miR-1388-5p mimics and pre-miR-1388 plasmids inhibit IRAK1 expression by complementing the seed sequence in the 3'-untranslated region (3'-UTR) of IRAK1. Finally, we observed that miR-1388-5p could negatively regulate NF-kappa B pathways through targeting IRAK1. These results provide new insights into the function of miR-1388-5p in fish innate immunity, meanwhile enriching miRNA-mediated regulatory networks.
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