期刊
DEVELOPMENT
卷 148, 期 12, 页码 -出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/dev.198754
关键词
Brain; Cell lineage; Dopaminergic cell; MAPK pathway; Otx; Ciona
资金
- Japan Society for the Promotion of Science (JSPS) [17K15130, 19K16150, 16H04724, 19H03213]
- Hirao Taro Foundation of KONAN GAKUEN for Academic Research
- Takeda Science Foundation [2015021209]
- Japan Science Society
- Grants-in-Aid for Scientific Research [16H04724, 17K15130, 19H03213, 19K16150] Funding Source: KAKEN
The dopaminergic cells in the Ciona larva are derived from a bilateral pair of cells called a9.37 cells at the center of the neural plate, with all descendants of the posterior daughter cells differentiating into dopaminergic cells. The MAPK pathway and the transcription factor Otx are required for the expression of Fer2 in the dopaminergic cell lineage, providing a cellular and molecular framework for understanding the commitment to dopaminergic cells in the simple chordate brain.
The Ciona larva has served as a unique model for understanding the development of dopaminergic cells at single-cell resolution owing to the exceptionally small number of neurons in its brain and its fixed cell lineage during embryogenesis. A recent study suggested that the transcription factors Fer2 and Meis directly regulate the dopamine synthesis genes in Ciona, but the dopaminergic cell lineage and the gene regulatory networks that control the development of dopaminergic cells have not been fully elucidated. Here, we reveal that the dopaminergic cells in Ciona are derived from a bilateral pair of cells called a9.37 cells at the center of the neural plate. The a9.37 cells divide along the anterior-posterior axis, and all of the descendants of the posterior daughter cells differentiate into the dopaminergic cells. We show that the MAPK pathway and the transcription factor Otx are required for the expression of Fer2 in the dopaminergic cell lineage. Our findings establish the cellular and molecular framework for fully understanding the commitment to dopaminergic cells in the simple chordate brain.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据