期刊
DEVELOPMENT
卷 148, 期 16, 页码 -出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/dev.199686
关键词
Autophagy; Mitochondrial dynamics; Mitophagy; Spermatid; Spermatogenesis; Mouse
资金
- National Institutes of Health [R35 GM127147]
- National Science Foundation Graduate Research Fellowship [DGE1144469]
- National Institutes of Health Cell and Molecular Biology Training Grant [GM07616T32]
- Japan Society for the Promotion of Science (JSPS) KAKENHI grant [16KK0162]
- JSPS KAKENHI grant [16H06276]
- National Institute of Allergy and Infectious Diseases (NIAID) [2 P50 AI150464]
- California Institute of Technology
- Grants-in-Aid for Scientific Research [16KK0162] Funding Source: KAKEN
Male germline development involves coordinated changes in mitochondrial number, morphology, and organization, with processes such as fusion, fission, and mitophagy playing important roles. Disruption of Fis1 in mouse male germline results in abnormal mitochondrial structure and increased content, leading to defects in spermatid maturation.
Male germline development involves choreographed changes to mitochondrial number, morphology and organization. Mitochondrial reorganization during spermatogenesis was recently shown to require mitochondrial fusion and fission. Mitophagy, the autophagic degradation of mitochondria, is another mechanism for controlling mitochondrial number and physiology, but its role during spermatogenesis is largely unknown. During post-meiotic spermatid development, restructuring of the mitochondrial network results in packing of mitochondria into a tight array in the sperm midpiece to fuel motility. Here, we show that disruption of mouse Fis1 in the male germline results in early spermatid arrest that is associated with increased mitochondrial content. Mutant spermatids coalesce into multinucleated giant cells that accumulate mitochondria of aberrant ultrastructure and numerous mitophagic and autophagic intermediates, suggesting a defect in mitophagy. We conclude that Fis1 regulates mitochondrial morphology and turnover to promote spermatid maturation.
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