期刊
CYTOLOGIA
卷 86, 期 2, 页码 149-154出版社
UNIV TOKYO CYTOLOGIA
DOI: 10.1508/cytologia.86.149
关键词
Nicotiana; Chromosome; FISH; rDNA; Phylogenesis
资金
- Open Foundation of Molecular Genetics Key Laboratory of China Tobacco (Guizhou Academy of Tobacco Science)
- Support Plan for Science and Technology Top-notch Talents of Guizhou Higher Education Institutions [Qianjiaohe KY zi (2016) 064]
Physical mapping of 18S and 5S rDNA sites in eight Nicotiana species using double-probe FISH revealed differences in chromosomal distribution among different species. These findings contribute to understanding the genome evolution of the genus Nicotiana and have implications for genetic breeding and germplasm innovation in tobacco.
Studying the genome evolution of Nicotiana has been hindered by the small chromosome and complex chromosomal number. Consequently, studying the chromosomal structure is a very important work in clarifying the genome evolution of genus Nicotiana. In the present study, the 18S and 5S rDNA sites of eight Nicotiana species were physically mapped by double-probe FISH. Results showed the following: 1) each species (including the S-genome and T-genome of N. tabacum) had one or two pairs of 5S rDNA sites and chromosome VIII of each species had one pair of 5S rDNA sites in the interstitial region of the long arm; 2) each species (including the S-genome and T-genome of N. tabacum) had one, two or three pairs of 18S rDNA sites; 3) the chromosomal distribution of 5S and 18S rDNA was same between N. setchellii, N. kawakamii and N. otophora, and between N. glutinosa and N. tomentosa; and the chromosomal distribution of 5S and 18S rDNA of N. sylvstris and N. tomentosiformis was same with the S-genome and T-genome of N. tabacum, respectively. Finally, the genome evolutions of the genus Nicotiana were inferred. The present results could provide obvious references for studies on the system and phylogenesis of genus Nicotiana and were of significance to genetic breeding and germplasm innovation for tobacco.
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