4.2 Review

Recent lessons learned for ex-vivo platelet production

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Article Hematology

Cytoskeletal-based mechanisms differently regulate in vivo and in vitro proplatelet formation

Alicia Bornert et al.

Summary: Platelets are produced through native proplatelets (nPPT) from bone marrow megakaryocytes, with the elongation of nPPT in living mice found to be in equilibrium between protrusion and retraction forces mediated by myosin-IIA. The behavior of microtubules differs between cPPT and nPPT in different mouse models, with microtubules playing a critical role in vitro but less so in vivo. Additionally, it was discovered that blood Stokes' forces may be sufficient to promote nPPT extension independently of myosin and microtubules, proposing a new mechanism for nPPT extension.

HAEMATOLOGICA (2021)

Article Multidisciplinary Sciences

P38 mitogen activated protein kinase inhibitor improves platelet in vitro parameters and in vivo survival in a SCID mouse model of transfusion for platelets stored at cold or temperature cycled conditions for 14 days

Andrey Skripchenko et al.

Summary: This study evaluated the effects of using p38MAPK inhibitor VX-702 to improve platelet storage in cold or thermocycling conditions, finding that VX-702 helped to improve platelet parameters and circulation.

PLOS ONE (2021)

Article Multidisciplinary Sciences

Development of an ex vivo xenogeneic bone environment producing human platelet-like cells

Shingo Fujiyama et al.

PLOS ONE (2020)

Article Immunology

Membrane budding is a major mechanism of in vivo platelet biogenesis

Kathryn S. Potts et al.

JOURNAL OF EXPERIMENTAL MEDICINE (2020)

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BRD4 directs hematopoietic stem cell development and modulates macrophage inflammatory responses

Anup Dey et al.

EMBO JOURNAL (2019)

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New Insights Into the Differentiation of Megakaryocytes From Hematopoietic Progenitors

Leila J. Noetzli et al.

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Mesenchymal stromal cells lower platelet activation and assist in platelet formation in vitro

Avital Mendelson et al.

JCI INSIGHT (2019)

Article Biochemistry & Molecular Biology

Turbulence Activates Platelet Biogenesis to Enable Clinical Scale Ex Vivo Production

Yukitaka Ito et al.

Review Hematology

Platelet Storage Lesions: What More Do We Know Now?

Monica Suet Ying Ng et al.

TRANSFUSION MEDICINE REVIEWS (2018)

Review Critical Care Medicine

Platelet storage duration and its clinical and transfusion outcomes: a systematic review\

Cecile Aubron et al.

CRITICAL CARE (2018)

Article Multidisciplinary Sciences

The lung is a site of platelet biogenesis and a reservoir for haematopoietic progenitors

Emma Lefrancais et al.

NATURE (2017)

Article Hematology

Continued decline in blood collection and transfusion in the United States-2015

Katherine D. Ellingson et al.

TRANSFUSION (2017)

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Microfluidic model of the platelet-generating organ: beyond bone marrow biomimetics

Antoine Blin et al.

SCIENTIFIC REPORTS (2016)

Article Medicine, Research & Experimental

Inducible Gata1 suppression expands megakaryocyte-erythroid progenitors from embryonic stem cells

Ji-Yoon Noh et al.

JOURNAL OF CLINICAL INVESTIGATION (2015)

Article Multidisciplinary Sciences

Platelet microparticles are internalized in neutrophils via the concerted activity of 12-lipoxygenase and secreted phospholipase A2-IIA

Anne-Claire Duchez et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2015)

Article Surgery

Changing Demographics of the American Population

Lhab Halaweish et al.

SURGICAL CLINICS OF NORTH AMERICA (2015)

Article Hematology

Platelet bioreactor-on-a-chip

Jonathan N. Thon et al.

Article Cell & Tissue Engineering

Scalable Generation of Universal Platelets from Human Induced Pluripotent Stem Cells

Qiang Feng et al.

STEM CELL REPORTS (2014)

Article Multidisciplinary Sciences

An Inhibition of p38 Mitogen Activated Protein Kinase Delays the Platelet Storage Lesion

Andrey Skripchenko et al.

PLOS ONE (2013)

Article Immunology

A novel role of sphingosine 1-phosphate receptor S1pr1 in mouse thrombopoiesis

Lin Zhang et al.

JOURNAL OF EXPERIMENTAL MEDICINE (2012)

Article Hematology

Prolonged continuous in vitro human platelet production using three-dimensional scaffolds

Brent Sullenbarger et al.

EXPERIMENTAL HEMATOLOGY (2009)

Article Multidisciplinary Sciences

Dynamic visualization of thrombopoiesis within bone marrow

Tobias Junt et al.

SCIENCE (2007)

Review Biochemistry & Molecular Biology

Phosphoinositide-metabolizing enzymes at the interface between membrane traffic and cell signalling

Michael Krauss et al.

EMBO REPORTS (2007)

Review Cardiac & Cardiovascular Systems

Activation of platelet function through G protein-coupled receptors

Stefan Offermanns

CIRCULATION RESEARCH (2006)

Article Biochemistry & Molecular Biology

Mechanisms of cold-induced platelet actin assembly

KM Hoffmeister et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2001)