Characterization of two novel heat-active α-galactosidases from thermophilic bacteria

Two genes (agal1 and agal2) encoding alpha-galactosidases were identified by sequence-based screening approaches. The gene agal1 was identified from a data set of a sequenced hot spring metagenome, and the deduced amino-acid sequence exhibited 99% identity to an alpha-galactosidase from the thermophilic bacterium Dictyoglomus thermophilum. The gene agal2 was identified from the whole genome sequence of the thermophile Meiothermus ruber. The amino-acid sequences exhibited structural motifs typical for glycoside hydrolase (GH) family 36 members and were also differentiated into different subgroups of this family. Recombinant production of the heat-active GH36b enzyme Agal1 (87 kDa) and GH36bt enzyme Agal2 (57 kDa) was carried out in E. coli. Agal1 exhibited a specific activity of 1502.3 U/mg at 80 A degrees C, pH 6.5, and Agal2 225.4 U/mg at 60-70 A degrees C, pH 6.5. Half-lives of 14 h (Agal1) and 39 h (Agal2) were obtained at 50 A degrees C, and Agal1 showed half-lives of 4 and 2 h at 70 and 80 A degrees C, respectively. In addition to the natural substrates melibiose, raffinose, and stachyose, 4NP alpha-d-galactopyranoside was hydrolyzed. Galactose was also liberated from locust bean gum. Both heat-active enzymes are attractive candidates for application in food and feed industry for high-temperature processes for the degradation of raffinose family oligosaccharides.