期刊
CROP PROTECTION
卷 151, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.cropro.2021.105824
关键词
Disease; Ora-pro-nobis; Unconventional food plants
类别
资金
- 'Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior-Brasil' (CAPES) [001]
- 'Conselho Nacional de Desenvolvimento Cientifico e Tecnologico' (CNPq) /Brazil
This study reported the discovery of Alternaria arborescens as the causal agent of leaf lesions in Pereskia aculeata in Curitiba, Brazil, marking the first global report of this pathogen infecting P. aculeata. The pathogen induced symptoms in plants, with all symptomatic leaves falling off 10 days post-inoculation, highlighting the importance for further research and conservation efforts for this plant species.
Pereskia aculeata, commonly known as ora-pro-nobis, is a is a highly nutritive species used for human consumption with potential of expansion. However, leaf lesions that did not match the symptoms and signs of previously described pathogenic species to this culture were observed in an ora-pro-nobis cultivation in Curitiba, Paran ' a state, southern Brazil. Therefore, the objectives of this study were to isolate, test the pathogenicity and characterize morphologically and molecularly the causal agent of the symptoms of this species. For this purpose, isolation from leaf fragments was performed. Pathogenicity was tested in potted cuttings which were wounded to simulate the thorns natural injuries and inoculated with a conidia suspension. The colony characteristics and morphological features of conidia were determined on V8 medium, and the molecular characterization was performed with Bayesian phylogenetic inference of the partial concatenated alignment of the ITS, gapdh and tef1 genes of the isolate. Morphological characterization matched the described for Alternaria spp. The symptoms on inoculated cuttings appeared within 4 days in all inoculated leaves, while the control plants remained asymptomatic, and 10 days after inoculation all symptomatic leaves fell from the plant. The characteristics of the re isolated fungus from the pathogenicity test lesions were morphologically similar to the isolate from naturally infected leaves. The sequences of the pathogen grouped in a reliable clade called Alternaria arborescens. To our knowledge, this is the first report of A. arborescens causing lesions on P. aculeata worldwide. Implications of these finding should be further studied.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据