期刊
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
卷 62, 期 32, 页码 8920-8934出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/10408398.2021.1937041
关键词
CRISPR; Cas technology; genetic engineering; microbial cell factories; polyunsaturated fatty acids; Yarrowia lipolytica
资金
- Nature Science Foundation of Jiangsu Province [BK20190706, 19KJB530011]
- National Natural Science Foundation of China [21908112, 22038007]
This article summarizes the recent advances in improving PUFAs production by Y. lipolytica through engineering strategies and bypass approaches, including optimizing biosynthetic pathways, enhancing precursor supply, and regulating competing pathways. In addition, the potential applications of CRISPR/Cas genome editing technology in Y. lipolytica for PUFAs production are further discussed.
Owing to the important physiological functions, polyunsaturated fatty acids (PUFAs) play a vital role in protecting human health, such as preventing cancer, cardiovascular disease, and diabetes. Specifically, Yarrowia lipolytica has been identified as the most popular non-conventional oleaginous yeast, which can accumulate the abundant intracellular lipids, indicating that has great potential as an industrial host for production of PUFAs. Notably, some novel engineering strategies have been applied to endow and improve the abilities of Y. lipolytica to synthesize PUFAs, including construction and optimization of PUFAs biosynthetic pathways, improvement of preucrsors acetyl-coA and NADPH supply, inhibition of competing pathways, knockout of beta-oxidation pathways, regulation of oxidative stress defense pathways, and regulation of genes involved in upstream lipid metabolism. Besides, some bypass approaches, such as strain mating, evolutionary engineering, and computational model based on omics, also have been proposed to improve the performance of engineering strains. Generally, in this review, we summarized the recent advances in engineering strategies and bypass approaches for improving PUFAs production by Y. lipolytica. In addition, we further summarized the latest efforts of CRISPR/Cas genome editing technology in Y. lipolytica, which is aimed to provide its potential applications in PUFAs production.
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