期刊
COMPUTATIONAL BIOLOGY AND CHEMISTRY
卷 92, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.compbiolchem.2021.107484
关键词
Antiangiogenic; Anticancer; Antioxidant activity; Flutamide; Autodock
资金
- Department of Sciences and Technology, New Delhi, India [SR/FT/CS019/2008]
- Department of Science and TechnologyPromotion of University Research and Scientific Excellence (DST-PURSE), India
- Rashtriya Uchchatar Shiksha Abhiyan (RUSA) Government of India
The synthesized N-(4-(substituted)-3-(trifluoromethyl) phenyl) isobutyramides and their N-ethyl analogues have shown promising anticancer activity, as well as dual inhibitory effects on angiogenesis and oxidative stress. The compounds 8 L, 8q, 9n, and 9p exhibited higher inhibitory activity on cancer cell viability than standard methotrexate, and also showed potential to inhibit proangiogenic cytokines. Additionally, in silico molecular docking data provided structural rationale for observed TNF alpha inhibition, highlighting the potential of these derivatives as anticancer agents with multiple mechanisms of action.
N-(4-(substituted)-3-(trifluoromethyl) phenyl) isobutyramides and their N-ethyl analogues (flutamides) are versatile scaffolds with a wide spectrum of biological activities. A series of new N-(4-(substituted)-3-(trifluoromethyl) phenyl) isobutyramides (8a-t) and their N-ethyl analogous (9a-t) were synthesized and characterized. The inhibitory potential of the synthesized compounds on the viability of three human cancer cell lines HEP3BPN 11 (liver), MDA-MB 453 (breast), and HL 60 (leukemia) were assessed. Among all the compounds 8 L, 8q, 9n and 9p showed higher inhibitory activity on the viability of HL 60 than the standard methotrexate. These lead molecules were then tested for their potential to inhibit the activity of proangiogenic cytokines. The compound 9n showed significantly better inhibition against two cytokines viz. TNF alpha and Leptin as compared to the standard suramin, while 9p has activity comparable to suramin against IGF1, VEGF, FGFb, and Leptin. The 8q is found to be strong antiangiogenic agent against IGF1, VEGF and TGF beta; while 8 L has showed activity against TNF alpha, VEGF, and Leptin inhibition. Furthermore antioxidant potential of 8a-t and 9a-t compounds was screened using DPPH, OH and SOR radical scavenging activities. The OH radical scavenging activity of 8c and DPPH activities of 9n as well as 9o are significant as compared to respective standards ascorbic acid and alpha-tocopherol. The 8c, 9p and 9 h have also exhibited potential antioxidant activity. Additionally, we present in silico molecular docking data to provide the structural rationale of observed TNF alpha inhibition against newly synthesized compounds. Overall, the synthesized flutamide derivatives have not only anticancer activity, but also possess dual inhibitory effect (anti-angiogenesis and antioxidant) and hence can act as a promising avenue to develop further anticancer agents.
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