4.4 Article

Combining of transcriptomic and proteomic data to mine immune-related genes and proteins in the liver of Cynoglossus semilaevis challenged with Vibrio anguillarum

出版社

ELSEVIER SCIENCE INC
DOI: 10.1016/j.cbd.2021.100864

关键词

Cynoglossus semilaevis; Vibrio anguillarum; Liver; Transcriptome; Proteome; Immunity

资金

  1. National Key Research and Development Program of China [2018YFD0900301-03]
  2. National Natural Science Foundation of China [41976102, 31572644]

向作者/读者索取更多资源

The study analyzed the transcriptomics and proteomics of the liver of Cynoglossus semilaevis infected with Vibrio anguillarum, revealing the significance of five key signaling pathways in enhancing liver immune defense. It identified important genes and proteins involved in immune response, recognition of pathogens, inflammatory response, and phagocytosis. The findings provide valuable information for further research on the mechanisms of liver immune response.
The liver is a multi-functional organ including metabolism, substance synthesis, detoxification, and various immune functions, and its role in immunity has attracted more and more attention. However, research on the liver immune response of fish infected by pathogenic bacteria is currently lacking. In this study, the transcriptomics and proteomics of the liver of Cynoglossus semilaevis infected with Vibrio anguillarum were analyzed. A total of 1470 genes and 497 proteins were differentially expressed in the pairwise comparison of obvious symptoms of infection (HOSG), no obvious symptoms of infection (NOSG) and PBS treatment (CG). Gene ontology and KEGG enrichment pathways analysis showed that differentially expressed genes (DEGs) and differentially expressed proteins (DEPs) were mainly enriched in toll-like receptors (TLRs), complement and coagulation cascades, nucleotide oligomerization domain (NOD)-like receptors (NLRs), mitogen-activated protein kinase (MAPK) and phagosome signaling pathways, which suggested the combined action of the five pathways were significant to enhance the liver immune defense. The combination of transcriptomic and proteomic analysis showed that ITG beta 1, C3, C5 and MRC1 were significantly up-regulated, which might play an important role in the liver immune response to the recognition of V. anguillarum, inflammatory response and phagocytosis. The transcriptome and proteome data we obtained provide information on some key genes and proteins for further study of the mechanism of liver immune response.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.4
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据