期刊
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS
卷 39, 期 -, 页码 -出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.cbd.2021.100844
关键词
Exosome; Proteomics; Shell; Nacre color; PRM; Hyriopsis cumingii
资金
- National Key Research and Development Program of China [2018YFD0901406]
- National Natural Science Foundation of China [31872565]
- Modern Agro-industry Technology Research System [CARS-49]
This study extracted exosomes from mantles of mussels with different nacre color, performed proteome sequencing analysis, and identified 758 differentially expressed proteins, including those involved in shell and nacre color formation. Validation experiments confirmed 14 differentially expressed proteins. This information enhances our understanding of the molecular mechanisms underlying shell and nacre color formation in Hyriopsis cumingii.
Pearl color is closely related to the nacre color of shell in Hyriopsis cumingii, and pearl color has a huge impact on its price. The nacre is an important part of the shell, and studies have suggested that mantle exosomes participated in shell formation. Exosomes contain many different proteins that are involved in different biological processes. In this study, exosomes were extracted from mantles of mussels with different nacre color. TMT quantitative proteome sequencing analysis was performed on purple and white mussel mantle exosomes, and 4861 proteins were obtained. Based on the standard of (|log2 (Fold change)| >= 1.2 or < 0.83 and p-value < 0.05), a total of 758 differentially expressed proteins were found. Some proteins involved in shell and nacre color formation were predicted with the proteins annotate, GO classification system. Moreover, 14 differentially expressed proteins (including eight up-regulated proteins and six down-regulated proteins) were validated using parallel reaction monitoring (PRM) assays. Overall, this information will be useful to improve our understanding of the molecular mechanisms of shell and nacre color formation in H. cumingii.
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