期刊
出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.cbpb.2021.110575
关键词
Hypoxia-inducible factor-1 alpha; Hypoxia; Monocytes/macrophages; Ayu; Phagocytosis; Bactericidal capacity
资金
- Program for the National Natural Science Foundation of China [41776151, 31972821]
- Zhejiang Provincial Natural Science Foundation of China [LR18C040001, LZ18C190001]
- Fundamental Research Funds for the Provincial Universities of Zhejiang [SJLZ2020002]
The study revealed that HIF-1 alpha in ayu plays a crucial role in the immune system by regulating cytokine expression, enhancing phagocytic and bactericidal activities, and improving host defenses.
Hypoxia-inducible factor-1 alpha (HIF-1 alpha) plays a critical role in immune and inflammatory responses and is important in controlling a variety of processes in monocytes and macrophages. However, the role of HIF-1 alpha in the teleost immune system remains less known. In this study, we cloned the cDNA sequence of HIF-1 alpha from the ayu (Plecoglossus altivelis, PaHIF-1 alpha). Sequence and phylogenetic tree analysis showed that PaHIF-1 alpha clustered within the fish HIF-1 alpha tree and was closely related to that of Northern pike (Esox Lucius). PaHIF-1 alpha was expressed in all tested tissues and expression increased in liver, head kidney, and body kidney upon Vibrio anguillarum infection. PaHIF-1 alpha was found to regulate the expression of cytokines in ayu monocytes/macrophages (MO/M Phi). PaHIF-1 alpha mediated hypoxia-induced enhancement of MO/M Phi phagocytic and bactericidal activities to enhance host defenses. Compared with the control, intermittent hypoxia further increased the expression of PaHIF-1 alpha mRNA, improved the survival rate, and reduced the bacterial load of V. anguillarum-infected ayu. Therefore, PaHIF-1 alpha may play a predominant role in the modulation of ayu MO/M Phi function.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据