期刊
出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.cbpa.2021.111078
关键词
Eriocheir sinensis; HMGR; Vitellogenesis; RNAi; Gene expression
资金
- Major Science and Technology Project of Anhui Province [202003b06020028]
- Fishery Industrial System of Anhui Province, China [[2021]711]
- Anhui Agricultural University College Students Innovation and Entrepreneurship Training Program Innovative Training Project Foundation [202110364005]
The study identified and characterized a full-length cDNA encoding HMGR in Eriocheir sinensis, which plays a potential role in vitellogenin biosynthesis in female crabs. RNA interference experiments showed that silencing of EsHMGR led to decreased expression of vitellogenin, suggesting its involvement in reproduction in crustaceans.
The sesquiterpenoid methyl farnesoate (MF), a de-epoxide form of insect juvenile hormone III (JH III), plays an essential role in regulating many crucial physiological processes in crustaceans including vitellogenesis and reproduction. 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR) is an important rate-limiting enzyme in the mevalonate pathway, which is critical for the synthesis of JH III and MF. In the present study, a full-length cDNA encoding HMGR (EsHMGR) in Eriocheir sinensis was isolated and characterised. Sequence analysis of EsHMGR revealed that it belongs to Class I HMGR family proteins with HMG-CoA-binding and NADPH-binding domains, both important for HMGR activity. In addition to its ubiquitous tissue expression, expression of EsHMGR was highly specific to the ovary, the main site of Vg synthesis. During ovarian development, EsHMGR expression in ovary displayed a stage-specific pattern, and was correlated with expression of vitellogenin (EsVg) in hepatopancreas, which suggests that EsHMGR possibly involved in vitellogenesis. To further investigate the functional role of EsHMGR in vitellogenin biosynthesis in E. sinensis, RNA interference-mediated gene silencing was carried out both in vitro and in vivo. Quantitative PCR results showed that injection of EsHMGR doublestranded RNA (dsRNA) led to a significant decrease in EsVg expression levels in ovary and hepatopancreas both in vitro and in vivo. Taken together, the results suggest that EsHMGR is involved in vitellogenin biosynthesis in female E. sinensis, which may provide a new resource for HMGR enzymes participating in reproduction in crustaceans.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据