期刊
CLINICAL ORAL INVESTIGATIONS
卷 26, 期 1, 页码 171-181出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00784-021-03988-4
关键词
Orthodontic tooth movement; Periodontitis; Fusobacterium nucleatum; Periodontium
资金
- German Society of Orthodontics (DGKFO), Sao Paulo Research Foundation, Brazil [FAPESP: 2014/20715-7, 2017/07137-2]
- German Research Foundation (DFG) [DE1593/5-1]
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES), Brazil, within the Program CAPES/PROBAL [88881.144012/2017-01]
- German Academic Exchange Service (DAAD) [57391253]
- Projekt DEAL
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [17/07137-2] Funding Source: FAPESP
The study found that periodontitis and orthodontic tooth movement can increase the expression levels of IL-6 and CXCL2, with mechanical strain enhancing the stimulatory effect of Fusobacterium nucleatum on IL-6.
Objectives The aim of this in vitro and in vivo study was to investigate the interaction of periodontitis and orthodontic tooth movement on interleukin (IL)-6 and C-X-C motif chemokine 2 (CXCL2). Materials and methods The effect of periodontitis and/or orthodontic tooth movement (OTM) on alveolar bone and gingival IL-6 and CXCL2 expressions was studied in rats by histology and RT-PCR, respectively. The animals were assigned to four groups (control, periodontitis, OTM, and combination of periodontitis and OTM). The IL-6 and CXCL2 levels were also studied in human gingival biopsies from periodontally healthy and periodontitis subjects by RT-PCR and immunohistochemistry. Additionally, the synthesis of IL-6 and CXCL2 in response to the periodontopathogen Fusobacterium nucleatum and/or mechanical strain was studied in periodontal fibroblasts by RT-PCR and ELISA. Results Periodontitis caused an increase in gingival levels of IL-6 and CXCL2 in the animal model. Moreover, orthodontic tooth movement further enhanced the bacteria-induced periodontal destruction and gingival IL-6 gene expression. Elevated IL-6 and CXCL2 gingival levels were also found in human periodontitis. Furthermore, mechanical strain increased the stimulatory effect of F. nucleatum on IL-6 protein in vitro. Conclusions Our study suggests that orthodontic tooth movement can enhance bacteria-induced periodontal inflammation and thus destruction and that IL-6 may play a pivotal role in this process.
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