期刊
CLINICAL ORAL INVESTIGATIONS
卷 26, 期 2, 页码 1569-1578出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00784-021-04130-0
关键词
Air-polishing; Periodontology; Cell biology; Glycine; Trehalose
资金
- Department of Periodontology and Operative Dentistry of the University Hospital of the Johannes-Gutenberg University, Germany
The study found that glycine powder significantly increased the expression of proinflammatory genes and exploited the NF-kappa B pathway, while trehalose powder hardly interfered with cell function and did not trigger the NF-kappa B pathway. In contrast to trehalose, glycine showed a significant inhibitory effect on the in vitro wound healing rate.
Objectives Air-polishing has been used in the treatment of periodontitis and gingivitis for years. The introduction of low-abrasive powders has enabled the use of air-polishing devices for subgingival therapy. Within the last decade, a wide range of different low-abrasive powders for subgingival use has been established. In this study, the effects of a glycine powder and a trehalose powder on human gingival fibroblasts (HGF) were investigated. Methods HGF were derived from three systemically and periodontally healthy donors. After 24 h and 48 h of incubation time, mRNA levels, and after 48 h, protein levels of TNF alpha, IL-8, CCL2, and VEGF were determined. In addition, NF-kappa B p65 nuclear translocation and in vitro wound healing were assessed. Statistical analysis was performed by ANOVA and post hoc Dunnett's and Tukey's tests (p < 0.05). Results Glycine powder significantly increased the expression of proinflammatory genes and showed exploitation of the NF-kappa B pathway, albeit trehalose powder hardly interfered with cell function and did not trigger the NF-kappa B pathway. In contrast to trehalose, glycine showed a significant inhibitory effect on the in vitro wound healing rate. Conclusion Subgingivally applicable powders for air-polishing devices can regulate cell viability and proliferation as well as cytokine expression. Our in vitro study suggests that the above powders may influence HGF via direct cell effects. Trehalose appears to be relatively inert compared to glycine powder.
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