期刊
CHINESE CHEMICAL LETTERS
卷 33, 期 1, 页码 293-297出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.cclet.2021.06.049
关键词
MoS2 nanosheets (NSs); 3D Caco-2 spheroids; Transcriptomics; Lipidomics; Fat digestion and absorption (map04975)
资金
- National Natural Science Foundation of China [51803055]
- Natural Science Foundation of Hunan Province [2019JJ50372]
- Hunan Provincial Key Research and Development Program [2018GK2062]
This study investigates the potential adverse effects of MoS2 nanosheets (NSs) and bulk materials on intestinal cells. The results show that MoS2 materials are non-cytotoxic but alter the lipid profiles in the cells.
MoS(2 )nanosheets (NSs) are novel 2D nanomaterials (NMs) with potential uses in many areas, and therefore oral exposure route to MoS2 NSs is plausible. Currently, MoS2 NSs are considered as biocompatible NMs, but there is lacking of systemic investigations to study the interactions of MoS2 NSs with intestinal cells. In this study, we exposed the 3D Caco-2 spheroids to MoS2 NSs or MoS2 powders (denoted as MoS2-bulk), and investigated the potential adverse effects of MoS2-materials based on transcriptomics and lipidomics analysis. As expected, both MoS(2)NSs and MoS2 -bulk were dose-dependently internalized into 3D Caco-2 spheroids but did not induce cytotoxicity, membrane disruption or decrease of thiols. However, the Gene Ontology (GO) and Kyoto Encyclopedia of Gene and Genomes (KEGG) analysis indicated that nutrient absorption and metabolism was decreased. One of the most significantly decreased KEGG pathways is fat digestion and absorption (map04975), and Western blotting analysis further showed that fatty acid binding protein 1 and apolipoprotein Al, key proteins involved in fat digestion and absorption, were down-regulated by MoS2 NSs or MoS2-bulk. In addition, BODIPY 493/503 staining suggested that exposure to MoS2 NSs and MoS2-bulk decreased lipid levels in the spheroids. However, lipidomics data indicated that MoS2 materials only decreased 8 lipid classes, including Iysophosphatidylcholine, Iysodimethylphosphatidylethanolamine, N-acylethanolamine, ceramide phosphoethanolamines, gangliosides, lysosphingomyelin and sulfatide, whereas most of the lipid classes were indeed increased. In addition, MoS2 NSs was more potent to decrease the lipid classes compared with MoS2 -bulk. Combined, the results from this study showed that MoS2 NSs and bulk materials were non-cytotoxic but altered lipid profiles in 3D Caco-2 spheroids. (C) 2021 Published by Elsevier B.V. on behalf of Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences.
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