期刊
CHEMSUSCHEM
卷 14, 期 15, 页码 3219-3225出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cssc.202100832
关键词
asymmetric catalysis; biocatalysis; cyanobacteria; ene-reduction; photocatalysis
资金
- European Union's Horizon 2020 research and innovation programme under the Marie Skodowska-Curie grant [764920]
- FET Open grant [899576]
This study demonstrates the use of internal illumination to overcome the cell density limitation of cyanobacterial biotransformations, achieving high reaction rates in a scalable photobioreactor.
Cyanobacteria have the capacity to use photosynthesis to fuel their metabolism, which makes them highly promising production systems for the sustainable production of chemicals. Yet, their dependency on visible light limits the cell-density, which is a challenge for the scale-up. Here, it was shown with the example of a light-dependent biotransformation that internal illumination in a bubble column reactor equipped with wireless light emitters (WLEs) could overcome this limitation. Cells of the cyanobacterium Synechocystis sp. PCC 6803 expressing the gene of the ene-reductase YqjM were used for the reduction of 2-methylmaleimide to (R)-2-methylsuccinimide with high optical purity (>99 % ee). Compared to external source of light, illumination by floating wireless light emitters allowed a more than two-fold rate increase. Under optimized conditions, product formation rates up to 3.7 mm h(-1) and specific activities of up to 65.5 U g(DCW)(-1) were obtained, allowing the reduction of 40 mm 2-methylmaleimide with 650 mg isolated enantiopure product (73 % yield). The results demonstrate the principle of internal illumination as a means to overcome the intrinsic cell density limitation of cyanobacterial biotransformations, obtaining high reaction rates in a scalable photobioreactor.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据