期刊
CHEMSUSCHEM
卷 14, 期 23, 页码 5235-5244出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cssc.202101492
关键词
biorefinery; green solvent; lignin; lignin depolymerization; sustainable process
资金
- National Science Foundation [CBET2027125]
- USDA National Institute of Food and Agriculture, McIntire Stennis project [1167926]
- U.S. Department of Energy (DOE) [DE-AC05-00OR22725]
- Center for Bioenergy Innovation (CBI), a U.S. DOE Bioenergy Research Center by the Office of Biological and Environmental Research in the DOE Office of Science
- U.S. Department of Energy, Office of Science, Office of Biological and Environmental Research [DE-AC02-05CH11231]
- Korea Institute of Science and Technology [2E31273]
Integrating plant genetic engineering with renewable deep eutectic solvents (DESs) led to the incorporation of PB-rich lignin into plant cell walls, resulting in enhanced release of fermentable sugars after DES pretreatment of mutant biomass. This approach shows promise for a sustainable closed-loop biorefinery system.
Integrating multidisciplinary research in plant genetic engineering and renewable deep eutectic solvents (DESs) can facilitate a sustainable and economic biorefinery. Herein, we leveraged a plant genetic engineering approach to specifically incorporate C6C1 monomers into the lignin structure. By expressing the bacterial ubiC gene in sorghum, p-hydroxybenzoic acid (PB)-rich lignin was incorporated into the plant cell wall while this monomer was completely absent in the lignin of the wild-type (WT) biomass. A DES was synthesized with choline chloride (ChCl) and PB and applied to the pretreatment of the PB-rich mutant biomass for a sustainable biorefinery. The release of fermentable sugars was significantly enhanced (similar to 190 % increase) compared to untreated biomass by the DES pretreatment. In particular, the glucose released from the pretreated mutant biomass was up to 12 % higher than that from the pretreated WT biomass. Lignin was effectively removed from the biomass with the preservation of more than half of the beta-omicron-4 linkages without condensed aromatic structures. Hydrogenolysis of the fractionated lignin was conducted to demonstrate the potential of phenolic compound production. In addition, a simple hydrothermal treatment could selectively extract PB from the same engineered lignin, showing a possible circular biorefinery. These results suggest that the combination of PB-based DES and engineered PB-rich biomass is a promising strategy to achieve a sustainable closed-loop biorefinery.
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