期刊
CHEMOSPHERE
卷 272, 期 -, 页码 -出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.chemosphere.2021.129863
关键词
Biochar; High-solid anaerobic digestion; Food waste; Cell viability; Methanogenic pathways
资金
- International Projects between Enterprises of Shanghai Science and Technology Commission (Shanghai - Singapore) [20230731400]
- National Key Research and Development Program of China [2019YFC1900602]
- National Research Foundation, Prime Minister's Office, Singapore under its Campus for Research Excellence and Technological Enterprise (CREATE) programme
The study found that an optimal dosage of biochar significantly enhances methane production in high-solid anaerobic digestion of food waste, particularly affecting butyric acid concentrations. The increase in cell viability due to the 25 g/L dosage of biochar contributes to the occurrence of direct interspecies electron transfer (DIET).
The underlying mechanisms of biochar enhance high-solid anaerobic digestion (HSAD) of food waste were investigated with a focus on the cell viability, microbial community, and methanogenic pathways. This study assessed the effects of different dosages of biochar in HSAD. Optimal biochar dosage was found to be 25 g/L, which produced accumulative methane yields of up to 251 mL CH4/g VS significantly promote volatile fatty acid degradations, especially in butyric acid concentrations. Effects of biochar with a dosage of 25 g/L on the cell viability showed that viable cells based on cell membrane integrity increased from 2.9% to 6.4%. Meanwhile, intact and highly active cells with high DNA content were probably involved in direct interspecies electron transfer (DIET) via membrane-bound electron transport proteins. Further analysis demonstrated that Syntrophomonas and methanogens Methanosarcina & Methanocelleus were selectively enriched by biochar, which resulted in the methanogenic pathways shifting from acetoclastic/hydrogenotrophic methanogenic pathways to more metabolically diverse methanogenic pathways. Accordingly, biochar-mediated DIET was possibly established between Syntrophomonas and Methanosarcina species due to those viable cells. In conclusion, biochar is a feasible additive in enhancing HSAD methanogenic performance. (C) 2021 Elsevier Ltd. All rights reserved.
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