期刊
CHEMICO-BIOLOGICAL INTERACTIONS
卷 344, 期 -, 页码 -出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.cbi.2021.109526
关键词
Steviol; Rebaudioside A; Steviolbioside; Albumin; Molecular docking analysis; Fluorescence spectroscopy
资金
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior - Brasil (CAPES) [001]
- Secretaria de Estado da Ciencia, Tecnologia e Ensino Superior (SETI) [6418/2017]
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)
The study found that steviol and its glycosides interact with bovine serum albumin, involving spontaneous binding reactions with hydrogen bonds and van der Waals interactions as well as water reorganization around the complex. The findings suggest that steviol's enhanced hydrophobicity and small size compared to its derivatives may favor its binding to BSA.
The interaction of the steviol and its glycosides (SG), steviolbioside, and rebaudioside A, with bovine serum albumin (BSA) was studied by absorption and fluorescence spectroscopy techniques alongside molecular docking. The stevia derivatives quenched the fluorescence of BSA by a dynamic quenching mechanism, indicating the interaction between the stevia derivatives and BSA. The binding constant (Kb) of steviol was 100-1000-fold higher than those of SG. The stevia derivative/BSA binding reaction was spontaneous and involved the formation of hydrogen bonds and van der Waals interactions between steviol and steviolbioside with BSA, and water reorganization around the rebaudioside A/BSA complex. Molecular docking pointed out the FA1 and FA9 binding sites of BSA as the probable binding sites of steviol and SG, respectively. In conclusion, steviol enhanced hydrophobicity and small size compared to SG may favor its binding to BSA. As steviol and its glycosides share binding sites on BSA with free fatty acids and drugs, they may be competitively displaced from plasma albumin under various physiological states or disease conditions. These findings are clinically relevant and provide an insight into the pharmacokinetics and pharmacodynamics of the stevia glycosides.
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