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Electrochemical droplet-based microfluidics using chip-based carbon paste electrodes for high-throughput analysis in pharmaceutical applications
Akkapol Suea-Ngam et al.
ANALYTICA CHIMICA ACTA (2015)
A Promiscuous De Novo Retro-Aldolase Catalyzes Asymmetric Michael Additions via Schiff Base Intermediates
Xavier Garrabou et al.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2015)
Expanding the Enzyme Universe: Accessing Non-Natural Reactions by Mechanism-Guided Directed Evolution
Hans Renata et al.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2015)
Evolution of Orbitrap Mass Spectrometry Instrumentation
Shannon Eliuk et al.
ANNUAL REVIEW OF ANALYTICAL CHEMISTRY, VOL 8 (2015)
Myoglobin-catalyzed intermolecular carbene N-H insertion with arylamine substrates
Gopeekrishnan Sreenilayam et al.
CHEMICAL COMMUNICATIONS (2015)
High throughput screening of enzyme activity with mass spectrometry imaging
Tristan de Rond et al.
CURRENT OPINION IN BIOTECHNOLOGY (2015)
Exometabolomics and MSI: deconstructing how cells interact to transform their small molecule environment
Leslie P. Silva et al.
CURRENT OPINION IN BIOTECHNOLOGY (2015)
Adeno-associated virus serotypes for gene therapeutics
Leszek Lisowski et al.
CURRENT OPINION IN PHARMACOLOGY (2015)
Metabolic engineering of Escherichia coli using CRISPR-Cas9 meditated genome editing
Yifan Li et al.
METABOLIC ENGINEERING (2015)
Rational design of a synthetic Entner-Doudoroff pathway for improved and controllable NADPH regeneration
Chiam Yu Ng et al.
METABOLIC ENGINEERING (2015)
Engineered CRISPR-Cas9 nucleases with altered PAM specificities
Benjamin P. Kleinstiver et al.
NATURE (2015)
Predicting the sequence specificities of DNA- and RNA-binding proteins by deep learning
Babak Alipanahi et al.
NATURE BIOTECHNOLOGY (2015)
Multiplexed tracking of combinatorial genomic mutations in engineered cell populations
Ramsey I. Zeitoun et al.
NATURE BIOTECHNOLOGY (2015)
Continuous directed evolution of DNA-binding proteins to improve TALEN specificity
Basil P. Hubbard et al.
NATURE METHODS (2015)
Methods for the directed evolution of proteins
Michael S. Packer et al.
NATURE REVIEWS GENETICS (2015)
Exploring the potential impact of an expanded genetic code on protein function
Han Xiao et al.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2015)
α-Enolase-binding peptide enhances drug delivery efficiency and therapeutic efficacy against colorectal cancer
Chien-Hsun Wu et al.
SCIENCE TRANSLATIONAL MEDICINE (2015)
Membrane transporter engineering in industrial biotechnology and whole cell biocatalysis
Douglas B. Kell et al.
TRENDS IN BIOTECHNOLOGY (2015)
Intermolecular carbene S-H insertion catalysed by engineered myoglobin-based catalysts
Vikas Tyagi et al.
CHEMICAL SCIENCE (2015)
Engineering a dirhodium artificial metalloenzyme for selective olefin cyclopropanation
Poonam Srivastava et al.
NATURE COMMUNICATIONS (2015)
Ultrahigh-throughput discovery of promiscuous enzymes by picodroplet functional metagenomics
Pierre-Yves Colin et al.
NATURE COMMUNICATIONS (2015)
Enzymatic C(sp(3))-H Amination: P450-Catalyzed Conversion of Carbonazidates into Oxazolidinones
Ritesh Singh et al.
ACS CATALYSIS (2015)
Enantioselective Enzyme-Catalyzed Aziridination Enabled by Active-Site Evolution of a Cytochrome P450
Christopher C. Farwell et al.
ACS CENTRAL SCIENCE (2015)
Highly Diastereoselective and Enantioselective Olefin Cyclopropanation Using Engineered Myoglobin-Based Catalysts
Melanie Bordeaux et al.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2015)
High Throughput Screening and Selection Methods for Directed Enzyme Evolution
Han Xiao et al.
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH (2015)
Structure Based Thermostability Prediction Models for Protein Single Point Mutations with Machine Learning Tools
Lei Jia et al.
PLOS ONE (2015)
Divergent evolution of a bifunctional de novo protein
Betsy A. Smith et al.
PROTEIN SCIENCE (2015)
RNAi-Assisted Genome Evolution in Saccharomyces cerevisiae for Complex Phenotype Engineering
Tong Si et al.
ACS SYNTHETIC BIOLOGY (2015)
Homology-Integrated CRISPR-Cas (HI-CRISPR) System for One-Step Multigene Disruption in Saccharomyces cerevisiae
Zehua Bao et al.
ACS SYNTHETIC BIOLOGY (2015)
Label-Free Measurements of Reaction Kinetics Using a Droplet-Based Optofluidic Device
Zhangming Mao et al.
JALA (2015)
Improved Cyclopropanation Activity of Histidine-Ligated Cytochrome P450 Enables the Enantioselective Formal Synthesis of Levomilnacipran
Z. Jane Wang et al.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2014)
Elevated Catalytic Activity of Ruthenium(II)-Porphyrin-Catalyzed Carbene/Nitrene Transfer and Insertion Reactions with N-Heterocyclic Carbene Ligands
Ka-Ho Chan et al.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2014)
Genome Engineering with Targetable Nucleases
Dana Carroll
ANNUAL REVIEW OF BIOCHEMISTRY, VOL 83 (2014)
mCSM: predicting the effects of mutations in proteins using graph-based signatures
Douglas E. V. Pires et al.
BIOINFORMATICS (2014)
Directed Evolution of a Cellodextrin Transporter for Improved Biofuel Production Under Anaerobic Conditions in Saccharomyces cerevisiae
Jiazhang Lian et al.
BIOTECHNOLOGY AND BIOENGINEERING (2014)
Genome-wide RNAi screen reveals the E3 SUMO-protein ligase gene SIZ1 as a novel determinant of furfural tolerance in Saccharomyces cerevisiae
Han Xiao et al.
BIOTECHNOLOGY FOR BIOFUELS (2014)
NeEMO: a method using residue interaction networks to improve prediction of protein stability upon mutation
Manuel Giollo et al.
BMC GENOMICS (2014)
A General Method for Artificial Metalloenzyme Formation through Strain-Promoted Azide-Alkyne Cycloaddition
Hao Yang et al.
CHEMBIOCHEM (2014)
Combinatorial Peptide Libraries: Mining for Cell-Binding Peptides
Bethany Powell Gray et al.
CHEMICAL REVIEWS (2014)
The enduring utility of continuous culturing in experimental evolution
David Gresham et al.
GENOMICS (2014)
An experimental framework for improved selection of binding proteins using SNAP display
Gillian Houlihan et al.
JOURNAL OF IMMUNOLOGICAL METHODS (2014)
Enzyme-Controlled Nitrogen-Atom Transfer Enables Regiodivergent C-H Amination
Todd K. Hyster et al.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2014)
Broccoli: Rapid Selection of an RNA Mimic of Green Fluorescent Protein by Fluorescence-Based Selection and Directed Evolution
Grigory S. Filonov et al.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2014)
Enantioselective lmidation of Sulfides via Enzyme-Catalyzed Intermolecular Nitrogen-Atom Transfer
Christopher C. Farwell et al.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2014)
Neutralizing the Detrimental Effect of Glutathione on Precious Metal Catalysts
Yvonne M. Wilson et al.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2014)
Directed evolution of genetic parts and circuits by compartmentalized partnered replication
Jared W. Ellefson et al.
NATURE BIOTECHNOLOGY (2014)
Negative selection and stringency modulation in phage-assisted continuous evolution
Jacob C. Carlson et al.
NATURE CHEMICAL BIOLOGY (2014)
Principles of genetic circuit design
Jennifer A. N. Brophy et al.
NATURE METHODS (2014)
Cryptic genetic variation: evolution's hidden substrate
Annalise B. Paaby et al.
NATURE REVIEWS GENETICS (2014)
Employing a combinatorial expression approach to characterize xylose utilization in Saccharomyces cerevisiae
Luke N. Latimer et al.
METABOLIC ENGINEERING (2014)
Impact of scaffold rigidity on the design and evolution of an artificial Diels-Alderase
Nathalie Preiswerk et al.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2014)
Evolution-guided optimization of biosynthetic pathways
Srivatsan Raman et al.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2014)
Genomically encoded analog memory with precise in vivo DNA writing in living cell populations
Fahim Farzadfard et al.
SCIENCE (2014)
Cytochrome P450-catalyzed insertion of carbenoids into N-H bonds
Z. Jane Wang et al.
CHEMICAL SCIENCE (2014)
Changing the peptide specificity of a human T-cell receptor by directed evolution
Sheena N. Smith et al.
NATURE COMMUNICATIONS (2014)
A system for the continuous directed evolution of proteases rapidly reveals drug-resistance mutations
Bryan C. Dickinson et al.
NATURE COMMUNICATIONS (2014)
P450-Catalyzed Intramolecular sp3 C-H Amination with Arylsulfonyl Azide Substrates
Ritesh Singh et al.
ACS CATALYSIS (2014)
Enhancing Tolerance to Short-Chain Alcohols by Engineering the Escherichia coli AcrB Efflux Pump to Secrete the Non-native Substrate n-Butanol
Michael A. Fisher et al.
ACS SYNTHETIC BIOLOGY (2014)
Lead Discovery for Human Kynurenine 3-Monooxygenase by High-Throughput RapidFire Mass Spectrometry
Denise M. Lowe et al.
JOURNAL OF BIOMOLECULAR SCREENING (2014)
Computational Enzyme Design
Gert Kiss et al.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2013)
Enhanced enantioselectivity of a carboxyl esterase from Rhodobacter sphaeroides by directed evolution
Jingbo Ma et al.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (2013)
Directed Evolution of a Highly Efficient Cellobiose Utilizing Pathway in an Industrial Saccharomyces cerevisiae strain
Yongbo Yuan et al.
BIOTECHNOLOGY AND BIOENGINEERING (2013)
Genome replication engineering assisted continuous evolution (GREACE) to improve microbial tolerance for biofuels production
Guodong Luan et al.
BIOTECHNOLOGY FOR BIOFUELS (2013)
Directed evolution of an E. coli inner membrane transporter for improved efflux of biofuel molecules
Jee Loon Foo et al.
BIOTECHNOLOGY FOR BIOFUELS (2013)
Chromosomal evolution of Escherichia coli for the efficient production of lycopene
Yun-Yan Chen et al.
BMC BIOTECHNOLOGY (2013)
Enzymatic reactions on immobilised substrates
Christopher J. Gray et al.
CHEMICAL SOCIETY REVIEWS (2013)
De novo enzymes by computational design
Hajo Kries et al.
CURRENT OPINION IN CHEMICAL BIOLOGY (2013)
Reporter-based screening and selection of enzymes
Teunke van Rossum et al.
FEBS JOURNAL (2013)
Representation Learning: A Review and New Perspectives
Yoshua Bengio et al.
IEEE TRANSACTIONS ON PATTERN ANALYSIS AND MACHINE INTELLIGENCE (2013)
What Makes a Protein Fold Amenable to Functional Innovation? Fold Polarity and Stability Trade-offs
Eynat Dellus-Gur et al.
JOURNAL OF MOLECULAR BIOLOGY (2013)
Enantioselective Synthesis of Cyclobutanes via Sequential Rh-catalyzed Bicyclobutanation/Cu-catalyzed Homoconjugate Addition
Robert Panish et al.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2013)
A Dual Anchoring Strategy for the Localization and Activation of Artificial Metalloenzymes Based on the Biotin-Streptavidin Technology
Jeremy M. Zimbron et al.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2013)
A multifunctional microfluidic droplet-array chip for analysis by electrospray ionization mass spectrometry
Yuan Su et al.
LAB ON A CHIP (2013)
Building synthetic gene circuits from combinatorial libraries: screening and selection strategies
Yolanda Schaerli et al.
MOLECULAR BIOSYSTEMS (2013)
Adrenaline-activated structure of β2-adrenoceptor stabilized by an engineered nanobody
Aaron M. Ring et al.
NATURE (2013)
Precision is essential for efficient catalysis in an evolved Kemp eliminase
Rebecca Blomberg et al.
NATURE (2013)
RNA-guided editing of bacterial genomes using CRISPR-Cas systems
Wenyan Jiang et al.
NATURE BIOTECHNOLOGY (2013)
A serine-substituted P450 catalyzes highly efficient carbene transfer to olefins in vivo
Pedro S. Coelho et al.
NATURE CHEMICAL BIOLOGY (2013)
Evolution of a designed retro-aldolase leads to complete active site remodeling
Lars Giger et al.
NATURE CHEMICAL BIOLOGY (2013)
Qualitative and quantitative metabolomic investigation of single neurons by capillary electrophoresis electrospray ionization mass spectrometry
Peter Nemes et al.
NATURE PROTOCOLS (2013)
In vitro selection of proteins with desired characteristics using mRNA-display
C. Alexander Valencia et al.
METHODS (2013)
Directed evolution of a cellobiose utilization pathway in Saccharomyces cerevisiae by simultaneously engineering multiple proteins
Dawn T. Eriksen et al.
MICROBIAL CELL FACTORIES (2013)
Targeted Drug Delivery Systems Mediated by a Novel Peptide in Breast Cancer Therapy and Imaging
Ruei-Min Lu et al.
PLOS ONE (2013)
Navigating the protein fitness landscape with Gaussian processes
Philip A. Romero et al.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2013)
Experimental interrogation of the path dependence and stochasticity of protein evolution using phage-assisted continuous evolution
Bryan C. Dickinson et al.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2013)
Engineering of TEV protease variants by yeast ER sequestration screening (YESS) of combinatorial libraries
Li Yi et al.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2013)
Olefin Cyclopropanation via Carbene Transfer Catalyzed by Engineered Cytochrome P450 Enzymes
Pedro S. Coelho et al.
SCIENCE (2013)
In Vivo-Directed Evolution of a New Adeno-Associated Virus for Therapeutic Outer Retinal Gene Delivery from the Vitreous
Deniz Dalkara et al.
SCIENCE TRANSLATIONAL MEDICINE (2013)
ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering
Thomas Gaj et al.
TRENDS IN BIOTECHNOLOGY (2013)
Artificial Metalloenzymes and Metallopeptide Catalysts for Organic Synthesis
Jared C. Lewis
ACS CATALYSIS (2013)
Yeast Oligo-Mediated Genome Engineering (YOGE)
James E. DiCarlo et al.
ACS SYNTHETIC BIOLOGY (2013)
Acoustic deposition with NIMS as a high-throughput enzyme activity assay
Matthew Greving et al.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY (2012)
Improved ethanol production by a xylose-fermenting recombinant yeast strain constructed through a modified genome shuffling method
Wei Zhang et al.
BIOTECHNOLOGY FOR BIOFUELS (2012)
Three-Dimensional Structures of Membrane Proteins from Genomic Sequencing
Thomas A. Hopf et al.
CELL (2012)
Engineering multicellular traits in synthetic microbial populations
John S. Chuang
CURRENT OPINION IN CHEMICAL BIOLOGY (2012)
Directed evolution of novel adeno-associated viruses for therapeutic gene delivery
M. A. Bartel et al.
GENE THERAPY (2012)
dRTP and dPTP a complementary nucleotide couple for the Sequence Saturation Mutagenesis (SeSaM) method
Anna Joelle Ruff et al.
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC (2012)
Increased Diels-Alderase activity through backbone remodeling guided by Foldit players
Christopher B. Eiben et al.
NATURE BIOTECHNOLOGY (2012)
Genome-scale promoter engineering by coselection MAGE
Harris H. Wang et al.
NATURE METHODS (2012)
High-throughput enzyme evolution in Saccharomyces cerevisiae using a synthetic RNA switch
Joshua K. Michener et al.
METABOLIC ENGINEERING (2012)
Enhanced multiplex genome engineering through co-operative oligonucleotide co-selection
Peter A. Carr et al.
NUCLEIC ACIDS RESEARCH (2012)
Customized optimization of metabolic pathways by combinatorial transcriptional engineering
Jing Du et al.
NUCLEIC ACIDS RESEARCH (2012)
Olefin Cyclopropanation Catalyzed by Iridium(III) Porphyrin Complexes
Bernie J. Anding et al.
ORGANOMETALLICS (2012)
PROTS-RF: A Robust Model for Predicting Mutation-Induced Protein Stability Changes
Yunqi Li et al.
PLOS ONE (2012)
Predicting the Functional Effect of Amino Acid Substitutions and Indels
Yongwook Choi et al.
PLOS ONE (2012)
Bridging the gaps in design methodologies by evolutionary optimization of the stability and proficiency of designed Kemp eliminase KE59
Olga Khersonsky et al.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2012)
The Crystal Structure of TAL Effector PthXo1 Bound to Its DNA Target
Amanda Nga-Sze Mak et al.
SCIENCE (2012)
Structural Basis for Sequence-Specific Recognition of DNA by TAL Effectors
Dong Deng et al.
SCIENCE (2012)
Biotinylated Rh(III) Complexes in Engineered Streptavidin for Accelerated Asymmetric C-H Activation
Todd K. Hyster et al.
SCIENCE (2012)
CAVER 3.0: A Tool for the Analysis of Transport Pathways in Dynamic Protein Structures
Eva Chovancova et al.
PLOS COMPUTATIONAL BIOLOGY (2012)
The Saccharomyces cerevisiae SCRaMbLE system and genome minimization
Jessica Dymond et al.
BIOENGINEERED (2012)
Retroviral Display in Gene Therapy, Protein Engineering, and Vaccine Development
Johannes H. Urban et al.
ACS CHEMICAL BIOLOGY (2011)
Laboratory Evolution of Stereoselective Enzymes: A Prolific Source of Catalysts for Asymmetric Reactions
Manfred T. Reetz
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2011)
OsO4 center dot Streptavidin: A Tunable Hybrid Catalyst for the Enantioselective cis-Dihydroxylation of Olefins
Valentin Koehler et al.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2011)
Artificial Transfer Hydrogenases for the Enantioselective Reduction of Cyclic Imines
Marc Duerrenberger et al.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2011)
Saccharomyces cerevisiae Genome Shuffling through Recursive Population Mating Leads to Improved Tolerance to Spent Sulfite Liquor
Dominic Pinel et al.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY (2011)
Improved Galactose Fermentation of Saccharomyces cerevisiae Through Inverse Metabolic Engineering
Ki-Sung Lee et al.
BIOTECHNOLOGY AND BIOENGINEERING (2011)
Improved Product-Per-Glucose Yields in P450-Dependent Propane Biotransformations Using Engineered Escherichia coli
Rudi Fasan et al.
BIOTECHNOLOGY AND BIOENGINEERING (2011)
Screening of cellulases for biofuel production: Online monitoring of the enzymatic hydrolysis of insoluble cellulose using high-throughput scattered light detection
Gernot Jaeger et al.
BIOTECHNOLOGY JOURNAL (2011)
Screening for Enzyme Activity In Turbid Suspensions with Scattered Light
Robert Huber et al.
BIOTECHNOLOGY PROGRESS (2011)
PoPMuSiC 2.1: a web server for the estimation of protein stability changes upon mutation and sequence optimality
Yves Dehouck et al.
BMC BIOINFORMATICS (2011)
SeSaM-Tv-II Generates a Protein Sequence Space that is Unobtainable by epPCR
Hemanshu Mundhada et al.
CHEMBIOCHEM (2011)
SNAP Dendrimers: Multivalent Protein Display on Dendrimer-Like DNA for Directed Evolution
Miriam Kaltenbach et al.
CHEMBIOCHEM (2011)
Artificial metalloenzymes for olefin metathesis based on the biotin-(strept)avidin technology
Cheikh Lo et al.
CHEMICAL COMMUNICATIONS (2011)
Synthetic inter-species cooperation of host and virus for targeted genetic evolution
Dokyun Na et al.
JOURNAL OF BIOTECHNOLOGY (2011)
Directed evolution of a thermophilic endoglucanase (Cel5A) into highly active Cel5A variants with an expanded temperature profile
Chaoning Liang et al.
JOURNAL OF BIOTECHNOLOGY (2011)
LigPlot+: Multiple Ligand-Protein Interaction Diagrams for Drug Discovery
Roman A. Laskowski et al.
JOURNAL OF CHEMICAL INFORMATION AND MODELING (2011)
Evolution of Three Human GPCRs for Higher Expression and Stability
Igor Dodevski et al.
JOURNAL OF MOLECULAR BIOLOGY (2011)
New insights into riboswitch regulation mechanisms
Laurene Bastet et al.
MOLECULAR MICROBIOLOGY (2011)
A system for the continuous directed evolution of biomolecules
Kevin M. Esvelt et al.
NATURE (2011)
Cryptic genetic variation promotes rapid evolutionary adaptation in an RNA enzyme
Eric J. Hayden et al.
NATURE (2011)
Synthetic chromosome arms function in yeast and generate phenotypic diversity by design
Jessica S. Dymond et al.
NATURE (2011)
Predicting mutation outcome from early stochastic variation in genetic interaction partners
Alejandro Burga et al.
NATURE (2011)
A nucleoside kinase as a dual selector for genetic switches and circuits
Yohei Tashiro et al.
NUCLEIC ACIDS RESEARCH (2011)
OmniChange: The Sequence Independent Method for Simultaneous Site-Saturation of Five Codons
Alexander Dennig et al.
PLOS ONE (2011)
A general strategy for the evolution of bond-forming enzymes using yeast display
Irwin Chen et al.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2011)
Sequential Establishment of Stripe Patterns in an Expanding Cell Population
Chenli Liu et al.
SCIENCE (2011)
Silver-Catalyzed C-C Bond Formation Between Methane and Ethyl Diazoacetate in Supercritical CO2
Ana Caballero et al.
SCIENCE (2011)
Precise Manipulation of Chromosomes in Vivo Enables Genome-Wide Codon Replacement
Farren J. Isaacs et al.
SCIENCE (2011)
High-Throughput Screening of Small Molecule Libraries using SAMDI Mass Spectrometry
Zachary A. Gurard-Levin et al.
ACS COMBINATORIAL SCIENCE (2011)
Predicting changes in protein thermostability brought about by single- or multi-site mutations
Jian Tian et al.
BMC BIOINFORMATICS (2010)
Natural Diversity to Guide Focused Directed Evolution
Helge Jochens et al.
CHEMBIOCHEM (2010)
High-Throughput Screening of Enzymes by Retroviral Display Using Droplet-Based Microfluidics
Lucia Granieri et al.
CHEMISTRY & BIOLOGY (2010)
Protein-based hybrid catalysts-design and evolution
Valentin Koehler et al.
CURRENT OPINION IN BIOTECHNOLOGY (2010)
The renaissance of continuous culture in the post-genomics age
Alan T. Bull
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY (2010)
Directed Evolution of an Enhanced and Highly Efficient FokI Cleavage Domain for Zinc Finger Nucleases
Jing Guo et al.
JOURNAL OF MOLECULAR BIOLOGY (2010)
Glycan Array on Aluminum Oxide-Coated Glass Slides through Phosphonate Chemistry
Shih-Huang Chang et al.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2010)
Iterative Saturation Mutagenesis Accelerates Laboratory Evolution of Enzyme Stereoselectivity: Rigorous Comparison with Traditional Methods
Manfred T. Reetz et al.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2010)
Rapid and Label-Free Screening of Enzyme Inhibitors Using Segmented Flow Electrospray Ionization Mass Spectrometry
Jian Pei et al.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY (2010)
Phenotypic robustness conferred by apparently redundant transcriptional enhancers
Nicolas Frankel et al.
NATURE (2010)
Rapid profiling of a microbial genome using mixtures of barcoded oligonucleotides
Joseph R. Warner et al.
NATURE BIOTECHNOLOGY (2010)
ConSurf 2010: calculating evolutionary conservation in sequence and structure of proteins and nucleic acids
Haim Ashkenazy et al.
NUCLEIC ACIDS RESEARCH (2010)
Development of an In Vitro Compartmentalization Screen for High-Throughput Directed Evolution of [FeFe] Hydrogenases
James A. Stapleton et al.
PLOS ONE (2010)
Induced allostery in the directed evolution of an enantioselective Baeyer-Villiger monooxygenase
Sheng Wu et al.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2010)
Ultrahigh-throughput screening in drop-based microfluidics for directed evolution
Jeremy J. Agresti et al.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2010)
The discovery of zinc fingers and their development for practical applications in gene regulation and genome manipulation
Aaron Klug
QUARTERLY REVIEWS OF BIOPHYSICS (2010)
Biocatalytic Asymmetric Synthesis of Chiral Amines from Ketones Applied to Sitagliptin Manufacture
Christopher K. Savile et al.
SCIENCE (2010)
Computational Design of an Enzyme Catalyst for a Stereoselective Bimolecular Diels-Alder Reaction
Justin B. Siegel et al.
SCIENCE (2010)
Analysis of Samples Stored as Individual Plugs in a Capillary by Electrospray Ionization Mass Spectrometry
Jian Pei et al.
ANALYTICAL CHEMISTRY (2009)
Coupling Microdroplet Microreactors with Mass Spectrometry: Reading the Contents of Single Droplets Online
Luis M. Fidalgo et al.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2009)
Genome Shuffling in Clostridium diolis DSM 15410 for Improved 1,3-Propanediol Production
Burkhard Otte et al.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY (2009)
Aptamers and riboswitches: perspectives in biotechnology
Julia E. Weigand et al.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (2009)
Fast and accurate predictions of protein stability changes upon mutations using statistical potentials and neural networks: PoPMuSiC-2.0
Yves Dehouck et al.
BIOINFORMATICS (2009)
Fluorophore Binding Aptamers as a Tool for RNA Visualization
Katja Eydeler et al.
BIOPHYSICAL JOURNAL (2009)
Genome shuffling: Progress and applications for phenotype improvement
Jixian Gong et al.
BIOTECHNOLOGY ADVANCES (2009)
Ultrahigh-Throughput FACS-Based Screening for Directed Enzyme Evolution
Guangyu Yang et al.
CHEMBIOCHEM (2009)
A pH-responsive riboregulator
Gal Nechooshtan et al.
GENES & DEVELOPMENT (2009)
Development of a liquid chromatography-based screening methodology for proteolytic enzyme activity
Nils Helge Schebb et al.
JOURNAL OF CHROMATOGRAPHY A (2009)
Programming cells by multiplex genome engineering and accelerated evolution
Harris H. Wang et al.
NATURE (2009)
Redesigning dehalogenase access tunnels as a strategy for degrading an anthropogenic substrate
Martina Pavlova et al.
NATURE CHEMICAL BIOLOGY (2009)
Enzymatic assembly of DNA molecules up to several hundred kilobases
Daniel G. Gibson et al.
NATURE METHODS (2009)
HotSpot Wizard: a web server for identification of hot spots in protein engineering
Antonin Pavelka et al.
NUCLEIC ACIDS RESEARCH (2009)
SITEHOUND-web: a server for ligand binding site identification in protein structures
Marylens Hernandez et al.
NUCLEIC ACIDS RESEARCH (2009)
A Novel Peptide Enhances Therapeutic Efficacy of Liposomal Anti-Cancer Drugs in Mice Models of Human Lung Cancer
De-Kuan Chang et al.
PLOS ONE (2009)
Golden Gate Shuffling: A One-Pot DNA Shuffling Method Based on Type IIs Restriction Enzymes
Carola Engler et al.
PLOS ONE (2009)
Circular Polymerase Extension Cloning of Complex Gene Libraries and Pathways
Jiayuan Quan et al.
PLOS ONE (2009)
Digital screening methodology for the directed evolution of transglycosidases
Fankroma M. T. Kone et al.
PROTEIN ENGINEERING DESIGN & SELECTION (2009)
Breaking the Code of DNA Binding Specificity of TAL-Type III Effectors
Jens Boch et al.
SCIENCE (2009)
A Simple Cipher Governs DNA Recognition by TAL Effectors
Matthew J. Moscou et al.
SCIENCE (2009)
Antigenic Fingerprinting of H5N1 Avian Influenza Using Convalescent Sera and Monoclonal Antibodies Reveals Potential Vaccine and Diagnostic Targets
Surender Khurana et al.
PLOS MEDICINE (2009)
A novel megaprimed and ligase-free, PCR-based, site-directed mutagenesis method
Wen-Chi Tseng et al.
ANALYTICAL BIOCHEMISTRY (2008)
Artificial metalloenzymes for asymmetric allylic alkylation on the basis of the biotin-avidin technology
Julien Pierron et al.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2008)
X-ray structure and designed evolution of an artificial transfer hydrogenase
Marc Creus et al.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2008)
Protein design by directed evolution
Christian Jaeckel et al.
ANNUAL REVIEW OF BIOPHYSICS (2008)
An efficient one-step site-directed deletion, insertion, single and multiple-site plasmid mutagenesis protocol
Huanting Liu et al.
BMC BIOTECHNOLOGY (2008)
A robust protein host for anchoring chelating ligands and organocatalysts
Manfred T. Reetz et al.
CHEMBIOCHEM (2008)
Constructing and Analyzing the Fitness Landscape of an Experimental Evolutionary Process
Manfred T. Reetz et al.
CHEMBIOCHEM (2008)
A novel genetic selection system for improved enantioselectivity of Bacillus subtilis lipase A
Ykelien L. Boersma et al.
CHEMBIOCHEM (2008)
Greatly reduced amino acid alphabets in directed evolution: making the right choice for saturation mutagenesis at homologous enzyme positions
Manfred T. Reetz et al.
CHEMICAL COMMUNICATIONS (2008)
Directed Evolution of a Lysosomal Enzyme with Enhanced Activity at Neutral pH by Mammalian Cell-Surface Display
Kai-Chuan Chen et al.
CHEMISTRY & BIOLOGY (2008)
Asymmetric catalytic cyclopropanation reactions in water
Irene Nicolas et al.
COORDINATION CHEMISTRY REVIEWS (2008)
Evolutionary History of a Specialized P450 Propane Monooxygenase
Rudi Fasan et al.
JOURNAL OF MOLECULAR BIOLOGY (2008)
Artificial metalloenzyme for enantioselective sulfoxidation based on vanadyl-loaded streptavidin
Anca Pordea et al.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2008)
AraC regulatory protein mutants with altered effector specificity
Shuang-Yan Tang et al.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2008)
Hepatocellular carcinoma cell-specific peptide ligand for targeted drug delivery
Albert Lo et al.
MOLECULAR CANCER THERAPEUTICS (2008)
Rapid Open-Source engineering of customized zinc-finger nucleases for highly efficient gene modification
Morgan L. Maeder et al.
MOLECULAR CELL (2008)
Kemp elimination catalysts by computational enzyme design
Daniela Rothlisberger et al.
NATURE (2008)
Catalytic C-H functionalization by metal carbenoid and nitrenoid insertion
Huw M. L. Davies et al.
NATURE (2008)
Directed enzyme evolution via small and effective neutral drift libraries
Rinkoo D. Gupta et al.
NATURE METHODS (2008)
Rates of evolutionary change in viruses: patterns and determinants
Siobain Duffy et al.
NATURE REVIEWS GENETICS (2008)
Catalytic C-H insertions using iron(III) porphyrin complexes
Hartun M. Mbuvi et al.
ORGANOMETALLICS (2008)
Directed evolution of a G protein-coupled receptor for expression, stability, and binding selectivity
Casim A. Sarkar et al.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2008)
A high-throughput microtiter plate-based screening method for the detection of full-length recombinant proteins
Matthias Mack et al.
PROTEIN EXPRESSION AND PURIFICATION (2008)
De novo computational design of retro-aldol enzymes
Lin Jiang et al.
SCIENCE (2008)
Transversion-enriched sequence saturation mutagenesis (SeSaM-Tv+): A random mutagenesis method with consecutive nucleotide exchanges that complements the bias of error-prone PCR
Tuck Seng Wong et al.
Biotechnology Journal (2007)
Protein engineers turned evolutionists
Sergio G. Peisajovich et al.
NATURE METHODS (2007)
Peptide-mediated targeting to tumor blood vessels of lung cancer for drug delivery
Tong-Young Lee et al.
CANCER RESEARCH (2007)
SELEX-A (r)evolutionary method to generate high-affinity nucleic acid ligands
Regina Stoltenburg et al.
BIOMOLECULAR ENGINEERING (2007)
Microtiter plates as mini-bioreactors: miniaturization of fermentation methods
Wouter A. Duetz
TRENDS IN MICROBIOLOGY (2007)
Pegaptanib and ranibizumab for neovascular age-related macular degeneration: a systematic review
A. L. Takeda et al.
BRITISH JOURNAL OF OPHTHALMOLOGY (2007)
A diverse family of thermostable cytochrome P450s created by recombination of stabilizing fragments
Yougen Li et al.
NATURE BIOTECHNOLOGY (2007)
Directed evolution can rapidly improve the activity of chimeric assembly-line enzymes
Michael A. Fischbach et al.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2007)
Dual selection of a genetic switch by a single selection marker
Yoko Nomura et al.
BIOSYSTEMS (2007)
Neutral genetic drift can alter promiscuous protein functions, potentially aiding functional evolution
Jesse D. Bloom et al.
BIOLOGY DIRECT (2007)
Top-down lipidomic screens by multivariate analysis of high-resolution survey mass spectra
Dominik Schwudke et al.
ANALYTICAL CHEMISTRY (2007)
Incorporating Synthetic Oligonucleotides via Gene Reassembiv (ISOR): a versatile tool forgenerating targeted libraries
Asael Herman et al.
PROTEIN ENGINEERING DESIGN & SELECTION (2007)
Improving catalytic function by ProSAR-driven enzyme evolution
Richard J. Fox et al.
NATURE BIOTECHNOLOGY (2007)
Iterative saturation mutagenesis (ISM) for rapid directed evolution of functional enzymes
Manfred T. Reetz et al.
NATURE PROTOCOLS (2007)
Enantiomerically enriched cyclopropene derivatives: Versatile building blocks in asymmetric synthesis
Ilan Marek et al.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2007)
Robustness-epistasis link shapes the fitness landscape of a randomly drifting protein
Shimon Bershtein et al.
NATURE (2006)
Three-membered-ring-based molecular architectures
Armin de Meijere et al.
CHEMICAL REVIEWS (2006)
A direct and efficient PAGE-mediated overlap extension PCR method for gene multiple-site mutagenesis
Ri-He Peng et al.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (2006)
The ancient Virus World and evolution of cells
Eugene V. Koonin et al.
BIOLOGY DIRECT (2006)
Combinatorial engineering of intergenic regions in operons tunes expression of multiple genes
Brian F. Pfleger et al.
NATURE BIOTECHNOLOGY (2006)
Selection of tumor-binding ligands in cancer patients with phage display libraries
David N. Krag et al.
CANCER RESEARCH (2006)
Pro-antibiotic substrates for the identification of enantioselective hydrolases
Bum-Yeol Hwang et al.
BIOTECHNOLOGY LETTERS (2006)
CUPSAT: prediction of protein stability upon point mutations
Vijaya Parthiban et al.
NUCLEIC ACIDS RESEARCH (2006)
CASTp: computed atlas of surface topography of proteins with structural and topographical mapping of functionally annotated residues
Joe Dundas et al.
NUCLEIC ACIDS RESEARCH (2006)
Dual selection enhances the signaling specificity of a variant of the quorum-sensing transcriptional activator LuxR
Cynthia H. Collins et al.
NATURE BIOTECHNOLOGY (2006)
Phage display systems and their applications
M Paschke
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (2006)
Microgram-scale testing of reaction conditions in solution using nanoliter plugs in microfluidics with detection by MALDI-MS
T Hatakeyama et al.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2006)
Dynamical principles of two-component genetic oscillators
Raul Guantes et al.
PLOS COMPUTATIONAL BIOLOGY (2006)
Prediction of protein stability changes for single-site mutations using support vector machines
JL Cheng et al.
PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS (2006)
Improved solubility of TEV protease by directed evolution
S van den Berg et al.
JOURNAL OF BIOTECHNOLOGY (2006)
Selection of biocatalysts for chemical synthesis
SV Fiet et al.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2006)
Directed evolution of adeno-associated virus yields enhanced gene delivery vectors
N Maheshri et al.
NATURE BIOTECHNOLOGY (2006)
Environmentally controlled invasion of cancer cells by engineered bacteria
JC Anderson et al.
JOURNAL OF MOLECULAR BIOLOGY (2006)
Error-prone rolling circle amplification: the simplest random mutagenesis protocol
Ryota Fujii et al.
NATURE PROTOCOLS (2006)
Iterative saturation mutagenesis on the basis of B factors as a strategy for increasing protein thermostability
Manfred T. Reetz et al.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2006)
Directed evolution of enantioselective enzymes: Iterative cycles of CASTing for probing protein-sequence space
MT Reetz et al.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2006)
High-throughput screening of enzyme libraries:: In vitro evolution of a β-galactosidase by fluorescence-activated sorting of double emulsions
E Mastrobattista et al.
CHEMISTRY & BIOLOGY (2005)
Development of a plasmid display system using GAL4 DNA binding domain for the in vitro screening of functional proteins
YS Choi et al.
BIOTECHNOLOGY LETTERS (2005)
Aqueous oxidation of alcohols catalyzed by artificial metalloenzymes based on the biotin-avidin technology
CM Thomas et al.
JOURNAL OF ORGANOMETALLIC CHEMISTRY (2005)
A rapid and efficient method for multiple-site mutagenesis with a modified overlap extension PCR
YF An et al.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (2005)
Structural basis for the enantioselectivity of an epoxide ring opening reaction catalyzed by halo alcohol dehalogenase HheC
RM de Jong et al.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2005)
Mimicking natural evolution in metallo-β-lactamases through second-shell ligand mutations
PE Tomatis et al.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2005)
Predicting protein stability changes from sequences using support vector machines
E Capriotti et al.
BIOINFORMATICS (2005)
Semi-rational approaches to engineering enzyme activity: combining the benefits of directed evolution and rational design
RA Chica et al.
CURRENT OPINION IN BIOTECHNOLOGY (2005)
I-Mutant2.0: predicting stability changes upon mutation from the protein sequence or structure
E Capriotti et al.
NUCLEIC ACIDS RESEARCH (2005)
Rapid creation of a novel protein function by in vitro coevolution
ZL Chen et al.
JOURNAL OF MOLECULAR BIOLOGY (2005)
Q-SiteFinder: an energy-based method for the prediction of protein-ligand binding sites
ATR Laurie et al.
BIOINFORMATICS (2005)
Directed evolution of specific receptor-ligand pairs for use in the creation of gene switches
K Chockalingam et al.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2005)
Reducing mutational bias in random protein libraries
T Vanhercke et al.
ANALYTICAL BIOCHEMISTRY (2005)
Tandem mass spectrometry for peptide and protein sequence analysis
JJ Coon et al.
BIOTECHNIQUES (2005)
Artificial metalloenzymes based on biotin-avidin technology for the enantioselective reduction of ketones by transfer hydrogenation
C Letondor et al.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2005)
Directed evolution of human T-cell receptors with picomolar affinities by phage display
Y Li et al.
NATURE BIOTECHNOLOGY (2005)
Structural genomics of GPCRs
K Lundstrom
TRENDS IN BIOTECHNOLOGY (2005)
High-throughput microcoil NMR of compound libraries using zero-dispersion segmented flow analysis
RA Kautz et al.
JOURNAL OF COMBINATORIAL CHEMISTRY (2005)
Reverse transcriptase and integrase of the Saccharomyces cerevisiae Ty1 element
FX Wilhelm et al.
CYTOGENETIC AND GENOME RESEARCH (2005)
Retrons, msDNA, and the bacterial genome
BC Lampson et al.
CYTOGENETIC AND GENOME RESEARCH (2005)
Tailoring the active site of chemzymes by using a chemogenetic-optimization procedure: Towards substrate-specific artificial hydrogenases based on the biotin-avidin technology
G Klein et al.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2005)
Directed in vitro evolution and crystallographic analysis of a peptide-binding single chain antibody fragment (scFv) with low picomolar affinity
C Zahnd et al.
JOURNAL OF BIOLOGICAL CHEMISTRY (2004)
Viral evolution as a tool to improve the tetracycline-regulated gene expression system
AT Das et al.
JOURNAL OF BIOLOGICAL CHEMISTRY (2004)
Sequence saturation mutagenesis (SeSaM): a novel method for directed evolution
TS Wong et al.
NUCLEIC ACIDS RESEARCH (2004)
Evolutionary potential of an RNA virus
EV Makeyev et al.
JOURNAL OF VIROLOGY (2004)
Multiple site-directed mutagenesis of more than 10 sites simultaneously and in a single round
A Seyfang et al.
ANALYTICAL BIOCHEMISTRY (2004)
Directed evolution of mammalian paraoxonases PON1 and PON3 for bacterial expression and catalytic specialization
A Aharoni et al.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2004)
Disease-specific B cell epitopes for serum antibodies from patients with severe acute respiratory syndrome (SARS) and serologic detection of SARS antibodies by epitope-based peptide antigens
IJ Liu et al.
JOURNAL OF INFECTIOUS DISEASES (2004)
Ultra-high-throughput screening based on cell-surface display and fluorescence-activated cell sorting for the identification of novel biocatalysts
S Becker et al.
CURRENT OPINION IN BIOTECHNOLOGY (2004)
Novel methods for directed evolution of enzymes: quality, not quantity
S Lutz et al.
CURRENT OPINION IN BIOTECHNOLOGY (2004)
Enantioselective biocatalysis optimized by directed evolution
KE Jaeger et al.
CURRENT OPINION IN BIOTECHNOLOGY (2004)
Pegaptanib for neovascular age-related macular degeneration
ES Gragoudas et al.
NEW ENGLAND JOURNAL OF MEDICINE (2004)
Assessing the likelihood of recurrence during RNA evolution in vitro
N Lehman
ARTIFICIAL LIFE (2004)
Evolution of new nonantibody proteins via iterative somatic hypermutation
L Wang et al.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2004)
Directed evolution of a glycosynthase via chemical complementation
HN Lin et al.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2004)
Inverse thinking about double mutants of enzymes
AS Mildvan
BIOCHEMISTRY (2004)
Artificial metalloenzymes: (Strept)avidin as host for enantioselective hydrogenation by achiral biotinylated rhodium-diphosphine complexes
M Skander et al.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2004)
Growth of Escherichia coli coexpressing phosphotriesterase and glycerophosphodiester phosphodiesterase, using paraoxon as the sole phosphorus source
SY McLoughlin et al.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY (2004)
One-step random mutagenesis by error-prone rolling circle amplification
R Fujii et al.
NUCLEIC ACIDS RESEARCH (2004)
A novel peptide specifically binding to nasopharyngeal carcinoma for targeted drug delivery
TY Lee et al.
CANCER RESEARCH (2004)
Identifying latent enzyme activities: Substrate ambiguity within modern bacterial sugar kinases
BG Miller et al.
BIOCHEMISTRY (2004)
Directed evolution of nucleic acid enzymes
GF Joyce
ANNUAL REVIEW OF BIOCHEMISTRY (2004)
WebLogo: A sequence logo generator
GE Crooks et al.
GENOME RESEARCH (2004)
Genome-wide somatic hypermutation
CL Wang et al.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2004)
Directed evolution of protein enzymes using nonhomologous random recombination
JA Bittker et al.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2004)
Enhanced levels of λ red-mediated recombinants in mismatch repair mutants
N Costantino et al.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2003)
PANTHER: A library of protein families and subfamilies indexed by function
PD Thomas et al.
GENOME RESEARCH (2003)
Continuous in vitro evolution of ribozymes that operate under conditions of extreme pH
H Kühne et al.
JOURNAL OF MOLECULAR EVOLUTION (2003)
Directed evolution of industrial enzymes: an update
JR Cherry et al.
CURRENT OPINION IN BIOTECHNOLOGY (2003)
Heterologous expression of G-protein-coupled receptors: comparison of expression systems from the standpoint of large-scale production and purification
V Sarramegna et al.
CELLULAR AND MOLECULAR LIFE SCIENCES (2003)
Evolutionary capacitance as a general feature of complex gene networks
A Bergman et al.
NATURE (2003)
General method for sequence-independent site-directed chimeragenesis
K Hiraga et al.
JOURNAL OF MOLECULAR BIOLOGY (2003)
SIFT: predicting amino acid changes that affect protein function
PC Ng et al.
NUCLEIC ACIDS RESEARCH (2003)
Engineering a mevalonate pathway in Escherichia coli for production of terpenoids
VJJ Martin et al.
NATURE BIOTECHNOLOGY (2003)
A colorimetric lead biosensor using DNAzyme-directed assembly of gold nanoparticles
JW Liu et al.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2003)
Automated nano-electrospray mass spectrometry for protein-ligand screening by noncovalent interaction applied to human H-FABP and A-FABP
K Benkestock et al.
JOURNAL OF BIOMOLECULAR SCREENING (2003)
Riboswitches control fundamental biochemical pathways in Bacillus subtilis and other bacteria
M Mandal et al.
CELL (2003)
Conditional gene expression by controlling translation with tetracycline-binding aptamers
B Suess et al.
NUCLEIC ACIDS RESEARCH (2003)
Stereoselective cyclopropanation reactions
H Lebel et al.
CHEMICAL REVIEWS (2003)
Somatic hypermutation of the B cell receptor genes B29 (Igβ, CD79b) and mb1 (Igα, CD79a)
MS Gordon et al.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2003)
Microbial cell-surface display
SY Lee et al.
TRENDS IN BIOTECHNOLOGY (2003)
Chemical complementation: A reaction-independent genetic assay for enzyme catalysis
K Baker et al.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2002)
Directed evolution of a genetic circuit
Y Yokobayashi et al.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2002)
Synthetic shuffling expands functional protein diversity by allowing amino acids to recombine independently
JE Ness et al.
NATURE BIOTECHNOLOGY (2002)
Generation and iterative affinity maturation of antibodies in vitro using hypermutating B-cell lines
SJ Cumbers et al.
NATURE BIOTECHNOLOGY (2002)
Reproducibility of oligonucleotide microarray transcriptome analyses -: An interlaboratory comparison using chemostat cultures of Saccharomyces cerevisiae
MDW Piper et al.
JOURNAL OF BIOLOGICAL CHEMISTRY (2002)
Directed evolution of high-affinity antibody mimics using mRNA display
LH Xu et al.
CHEMISTRY & BIOLOGY (2002)
Predicting changes in the stability of proteins and protein complexes: A study of more than 1000 mutations
R Guerois et al.
JOURNAL OF MOLECULAR BIOLOGY (2002)
Protein building blocks preserved by recombination
CA Voigt et al.
NATURE STRUCTURAL BIOLOGY (2002)
Genome shuffling of Lactobacillus for improved acid tolerance
R Patnaik et al.
NATURE BIOTECHNOLOGY (2002)
A monomeric red fluorescent protein
RE Campbell et al.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2002)
Directed evolution of enzymes and pathways for industrial biocatalysis
HM Zhao et al.
CURRENT OPINION IN BIOTECHNOLOGY (2002)
Somatic hypermutation of immunoglobulin genes: Merging mechanisms for genetic diversity
FN Papavasiliou et al.
CELL (2002)
Analysis of shuffled gene libraries
JM Joern et al.
JOURNAL OF MOLECULAR BIOLOGY (2002)
Genome shuffling leads to rapid phenotypic improvement in bacteria
YX Zhang et al.
NATURE (2002)
Steps toward mapping the human vasculature by phage display
W Arap et al.
NATURE MEDICINE (2002)
Random insertion and deletion of arbitrary number of bases for codon-based random mutation of DNAs
H Murakami et al.
NATURE BIOTECHNOLOGY (2002)
A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications
T Nagai et al.
NATURE BIOTECHNOLOGY (2002)
Rapidly maturing variants of the Discosoma red fluorescent protein (DsRed)
BJ Bevis et al.
NATURE BIOTECHNOLOGY (2002)
In vitro adaptation of a ligase ribozyme for activity under a low-pH condition
Y Miyamoto et al.
BIOTECHNOLOGY AND BIOENGINEERING (2001)
Identification of peptide sequences specific for serum antibodies from human papillomavirus-infected patients using phage display libraries
H Santamaria et al.
CLINICAL IMMUNOLOGY (2001)
Directed evolution of single proteins, metabolic pathways, and viruses
C Schmidt-Dannert
BIOCHEMISTRY (2001)
Biopanning and rapid analysis of selective interactive ligands
RJ Giordano et al.
NATURE MEDICINE (2001)
GFP-like chromoproteins as a source of far-red fluorescent proteins
NG Gurskaya et al.
FEBS LETTERS (2001)
Creating multiple-crossover DNA libraries independent of sequence identity
S Lutz et al.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2001)
Laboratory evolution of toluene dioxygenase to accept 4-picoline as a substrate
T Sakamoto et al.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY (2001)
Rapid evolution of novel traits in microorganisms
O Selifonova et al.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY (2001)
High efficiency mutagenesis, repair, and engineering of chromosomal DNA using single-stranded oligonucleotides
HM Ellis et al.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2001)
DNA synthesis fidelity by the reverse transcriptase of the yeast retrotransposon Ty1
M Boutabout et al.
NUCLEIC ACIDS RESEARCH (2001)
Libraries of hybrid proteins from distantly related sequences
V Sieber et al.
NATURE BIOTECHNOLOGY (2001)
Application of a very high-throughput digital imaging screen to evolve the enzyme galactose oxidase
S Delagrave et al.
PROTEIN ENGINEERING (2001)
DNA shuffling method for generating highly recombined genes and evolved enzymes
WM Coco et al.
NATURE BIOTECHNOLOGY (2001)
Techniques for increasing the throughput of flow injection mass spectrometry
KL Morand et al.
ANALYTICAL CHEMISTRY (2001)
Combinatorial and computational challenges for biocatalyst design
FH Arnold
NATURE (2001)
Applications of display technology in protein analysis
M Li
NATURE BIOTECHNOLOGY (2000)
A highly sensitive and selective catalytic DNA biosensor for lead ions
J Li et al.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2000)
Biochemistry, mutagenesis, and oligomerization of DsRed, a red fluorescent protein from coral
GS Baird et al.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2000)
High efficiency family shuffling based on multi-step PCR and in vivo DNA recombination in yeast: statistical and functional analysis of a combinatorial library between human cytochrome P450 1A1 and 1A2
Valerie Abecassis et al.
NUCLEIC ACIDS RESEARCH (2000)
Systemic sclerosis immunoglobulin G autoantibodies bind the human cytomegalovirus late protein UL94 and induce apoptosis in human endothelial cells
C Lunardi et al.
NATURE MEDICINE (2000)
Directed evolution of antibody fragments with monovalent femtomolar antigen-binding affinity
ET Boder et al.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2000)
Directed evolution of a stable scaffold for T-cell receptor engineering
EV Shusta et al.
NATURE BIOTECHNOLOGY (2000)
Molecular breeding of carotenoid biosynthetic pathways
C Schmidt-Dannert et al.
NATURE BIOTECHNOLOGY (2000)
Selection of peptides with semiconductor binding specificity for directed nanocrystal assembly
SR Whaley et al.
NATURE (2000)
Measuring the peptides in individual organelles with mass spectrometry
SS Rubakhin et al.
NATURE BIOTECHNOLOGY (2000)
Bacterial cell surface display of an enzyme library for selective screening of improved cellulase variants
YS Kim et al.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY (2000)
Exiting an RNA world
P Schimmel et al.
NATURE STRUCTURAL BIOLOGY (2000)
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