期刊
CHEMICAL ENGINEERING JOURNAL
卷 416, 期 -, 页码 -出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.cej.2021.129129
关键词
Electroactive microspheres; Aniline tetramer; DOPA-IGF-1; Osteogenic differentiation; Calvarial defect repair
资金
- Jilin Scientific and Technological Development Program [20200404110YY]
Electroactive microsphere systems have great potential for bone regeneration, especially in repairing challenging cases like skull defects. Combining with recombinant insulin-like growth-factor-1 containing 3,4-hydroxyphenalyalanine can further enhance mineralization and osteogenic differentiation, showing significant promise in calvarial defect repair.
Owing to defect features (irregular shape and size) and local environments (e.g., pulsation of dura matter and proximity of the brain), the skull is one of the most difficult to repair. Electroactive microsphere systems with good injectability, adjustable size, high surface-to-volume ratio and surface modifiability, exhibit excellent bone regeneration potential. Herein, the electroactive microspheres were prepared by immobilizing aniline tetramer (AT) on poly(lactic-co-glycolic acid)/hydroxyapatite (PLGA/HA) microspheres. Subsequently, the 3,4-hydroxyphenalyalanine-containing recombinant insulin-like growth-factor-1 (DOPA-IGF-1) inspired by bioorthogonal chemistry was designed by combining the recombinant DNA technology and tyrosinase treatment, and modified on electroactive scaffolds surface. The as-prepared microspheres exhibited excellent sphericity and homogeneity with an average diameter of 329.2 ? 16.5 ?m. Interestingly, the electroactive microsphere exhibits significant cell proliferation and enhanced osteogenic differentiation, and combining with DOPA-IGF-1 can further synergistically induce mineralization and osteogenic differentiation. Rat calvarial defect repair experiments showed excellent repairability in forming mineralized collagen deposition within the transplanted composite microspheres scaffold in the bone defect site. Thus, biomimetic composite microsphere with electroactivity and bioactivity exhibits considerable potential for calvarial defect repair.
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