期刊
CHEMICAL ENGINEERING JOURNAL
卷 420, 期 -, 页码 -出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.cej.2021.129703
关键词
PLGA porous microspheres; Silk coating; Biomineralization; Cell perfusion; hPDLSCs; Periodontal regeneration
资金
- CAMS Innovation Fund for Medical Sciences [2017I2M1007]
- National Natural Science Foundation of China [81670953]
- Tianjin Research Program for Distinguished Young Scholar [18JCJQJC48100]
- Peking Union Medical College [201920201501]
- China National Intellectual Property Administration [ZL201920247298.4]
This study focused on the potential of polymer porous microspheres (PMs) as stem cells delivery carriers for periodontal regeneration. The synthesized PLGA PMs, along with SF and/or HA modifications, were used to seed hPDLSCs with specific techniques. In vitro evaluations showed promising results for cell viability, migration, proliferation, and osteogenic differentiation. In vivo experiments demonstrated the superior tissue repairing efficacy of the HA-SF-PLGA group due to SF-dependent adhesion and HA-dependent osteogenesis.
Periodontitis, a widespread disease, is the main cause of tooth loss in adults, and there is no ideal clinical therapy for patients with severe alveolar bone defects. Research investigating the potential of polymer porous microspheres (PMs) as stem cells delivery carriers has been a hot-spot topic for tissue engineering in recent years. However, to date, it remains largely unknown regarding application of PMs in periodontal regeneration with satisfied cell seeding efficiency. In this study, PLGA PMs and corresponding silk fibroin (SF) or / and hydroxyapatite (HA) modified PLGA counterparts were synthesized by double emulsion-solvent evaporation, desolvation and biomineralization. Then, human periodontal ligament stem cells (hPDLSCs) were seeded into these carriers with a specific cell perfusion technique. Cell viability and migration, proliferation as well as osteogenic differentiation properties of the hPDLSCs were evaluated in vitro. In vivo experiment examining the impact of hPDLSCs suspension group and four cell delivery carriers groups on rat periodontal tissue regeneration further suggested that the HA-SF-PLGA group displayed superior tissue repairing efficacy due to SF-dependent high adhesion and HA-dependent osteogenesis induction. This novel tissue regeneration strategy based on PLGA hybrid PMs combined with a specific cell perfusion technique present promising potential of local stem cell delivery for effective clinical periodontitis therapy development, which is also applicable to tissue regeneration in other body parts.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据