期刊
CELLULAR AND MOLECULAR BIOENGINEERING
卷 15, 期 1, 页码 15-29出版社
SPRINGER
DOI: 10.1007/s12195-021-00697-6
关键词
Triple-negative breast cancer; Intravasation; Lymphovascular invasion; Microphysiological system; Tumor engineering; Fat
资金
- National Cancer Institute [U01 CA214292]
- National Institute of Diabetes and Digestive and Kidney Diseases [P30 DK046200]
- National Institute of General Medical Sciences [T32 GM008764]
- CURE Diversity Research Supplements Program at the National Cancer Institute
The study revealed that both adipocytes and adipose-derived stem cells accelerate the interstitial invasion and escape of human breast cancer cells, with ASCs having a stronger effect than adipocytes.
Introduction-Approximately 20-25% of human breast tumors are found within an adipose, rather than fibrous, stroma. Adipose stroma is associated with an increased risk of lymph node metastasis, but the causal association between adipose stroma and metastatic progression in human breast cancer remains unclear. Methods-We used micropatterned type I collagen gels to engineer similar to 3-mm-long microscale human breast tumors within a stroma that contains adipocytes and adipose-derived stem cells (ASCs) (collectively, adipose cells). Invasion and escape of human breast cancer cells into an empty 120-mu m-diameter lymphatic-like cavity was used to model interstitial invasion and vascular escape in the presence of adipose cell-derived factors for up to 16 days. Results-We found that adipose cells hasten invasion and escape by 1-2 days and 2-3 days, respectively. These effects were mediated by soluble factors secreted by the adipose cells, and these factors acted directly on tumor cells. Surprisingly, tumor invasion and escape were more strongly induced by ASCs than by adipocytes. Conclusions-This work reveals that both adipocytes and ASCs accelerate the interstitial invasion and escape of human breast cancer cells, and sheds light on the link between adipose stroma and lymphatic metastasis in human breast cancer.
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