期刊
CELLS TISSUES ORGANS
卷 211, 期 5, 页码 565-576出版社
KARGER
DOI: 10.1159/000518402
关键词
Follicle; Human bone marrow mesenchymal stem cells; In vitro culture; Vitrification
The effect of human bone marrow mesenchymal stem cells conditioned medium (hBMSCs-CM) on the growth and maturation of mouse ovarian follicles and embryonic development after vitrification was evaluated. The results showed that the presence of 7.5% CM significantly promoted antrum formation, oocyte maturation, and hormone secretion. Post-vitrification, the addition of 7.5% CM showed better results in terms of follicle developmental rate. However, there were no significant changes in fertilization and embryonic development rates.
The aim of this research study is to evaluate the effect of human bone marrow mesenchymal stem cells conditioned medium (hBMSCs-CM) on growth and maturation of mouse ovarian follicle, and embryonic development after vitrification. The hBMSCs were cultured, and the derived CM was collected, concentrated, and stored. 14-day-old mice ovaries were collected and randomly divided into vitrified and non-vitrified groups. Then their isolated preantral follicles were cultured for 12 days in alpha-MEM supplemented with different concentrations of CM (2.5, 5, and 7.5%). Finally, the growth and diameter of follicles, maturation of oocytes, hormone level, and embryo developmental rate were assessed. The results showed the antrum formation, oocyte maturation, and hormone secretion were significantly higher in the presence of 7.5% CM (p < 0.001). In the vitrified group, the developmental rate of follicles was lower than the non-vitrified group, and the subgroup containing 7.5% CM showed better results than the 5%, and 2.5% CM subgroups. However, no changes in fertilization and embryonic development rates were observed. Supplementing follicle culture media with 7.5% CM could enhance follicle growth and oocyte maturation of follicles after vitrification.
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