期刊
CARBON
卷 180, 期 -, 页码 67-76出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.carbon.2021.04.081
关键词
Graphene oxide; Fullerene; Cytotoxicity; Membrane-active peptide; Lipid packing state
资金
- National Natural Science Foundation of China [21774092, U1532108, U1932121]
- Priority Academic Program Development of Jiangsu Higher Education Institutions of Jiangsu Higher Education Institutions
The study demonstrates that low-concentration C-60 and GO can still mechanically perturb cell membranes, affecting the function realization of membrane active proteins/peptides. The impact of nanomaterials on cell membrane structures depends on particle properties and membrane environments.
Carbon nanomaterials such as fullerenes (C-60) and graphene oxide (GO) are considered as promising candidates for diverse applications in biotechnology and biomedicine. However, their potential toxic effects are still under debate. Herein, by using melittin (Mel), a representative pore-forming peptide, as a testing molecule we demonstrated that even the low-concentrated (usually assumed non-toxic) C-60 and GO could still mechanically perturb a cell membrane by adsorption and insertion, and consequently influence the function realization of membrane active proteins/peptides. Such perturbations, however, are particle-property and membrane-environment dependent. GO would sensitize both model bilayers and bacterial membranes to Mel, demonstrated as significantly enhanced membrane permeabilization ability or improved antibacterial performance of Mel. In contrast, C-60 activates the permeabilization effect of Mel on model membranes, while produces exactly the reverse effect on living bacteria and mammalian cells. Simulations further provide molecular details of the structural disturbance and probe the residue-specific formation of C-60-Mel complex in membrane. This work emphasizes the dependence of biological toxicity of nanomaterials on their physico-chemical properties, provides a facile method to detect the subtle structural perturbation of cell membranes at nanoscale, and suggests a necessity for a careful evaluation of the potential influences of nanomaterials on biological processes. (C) 2021 Elsevier Ltd. All rights reserved.
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