Structural analysis and biological effects of a neutral polysaccharide from the fruits of Rosa laevigata

A neutral water-soluble polysaccharide (RLP50-2) was extracted and purified from the fruits of Rosa laevigata. The absolute molecular weight was determined as 1.26 x 104 g/mol. Monosaccharide composition analysis showed that RLP50-2 mainly consisted of glucose, arabinose, and galactose. Structural analysis revealed that RLP50-2 consisted of -*5)-alpha-L-Araf-(1-*, -*2,5)-alpha-L-Araf-(1-*, -*3,5)-alpha-L-Araf-(1-*, -*4)-alpha-D-Glcp-(1-*, -*6)-alpha-DGlcp-(1-*, -*3,6)-beta-D-Glcp-(1-*, -*4)-alpha-D-Galp-(1-*, -*6)-beta-D-Galp-(1-*, -*2)-beta-D-Xylp-(1-*, terminal alpha-L-arabinose, and terminal beta-D-mannose. Biological assays showed that RLP50-2 had immunomodulatory activities using cell and zebrafish models. Moreover, RLP50-2 showed significantly antitumor activities by inhibiting tumor cell proliferation and migration and blocking angiogenesis. These results suggested that RLP50-2 could be developed as a potential immunomodulatory agent or antitumor candidate drug in biomedicine field.

Automated summary

A neutral water-soluble polysaccharide (RLP50-2) was extracted and purified from Rosa laevigata fruits, with monosaccharide composition mainly consisting of glucose, arabinose, and galactose. Structural analysis revealed specific glycosidic linkages and terminal constituents. Biological assays demonstrated immunomodulatory and antitumor activities of RLP50-2, suggesting its potential as a drug candidate in biomedicine.