期刊
CARBOHYDRATE POLYMERS
卷 264, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2021.118048
关键词
Hybrid hydrogels; Hyaluronic acid; Bioinspired scaffold; Nano-hydroxyapatite; Stem cells differentiation
资金
- National Natural Science Foundation of China [31771049]
- Foundation of key R&D Project of Jiangsu Province [BE2018731]
- Research Foundation of State Key Laboratory of Materials-Oriented Chemical Engineering [ZK201806, KL18-06]
- Six Talent Peaks Project of Jiangsu Province [SWYY-046]
The study introduces a hybrid hydrogel scaffold mimicking the microenvironment of osteochondral niches, effectively addressing the challenge of integrated repair of osteochondral tissue. The hydrogel exhibits excellent cytocompatibility and promotes cell survival and expression of genes related to bone and cartilage.
Aiming at the difficulty of integrated repair of osteochondral tissue, we designed a hybrid hydrogel scaffold that mimicked the microenvironment of osteochondral niches. Besides, the nano-hydroxyapatite (nHAP) was specially introduced into the hydrogel for its natural ability to promote bone regeneration. The hydrogel also exhibited good toughness (7500 KJ/m3), strength (1000 kPa), viscoelasticity, and in vitro cell experiments showed that hydrogels had quite good cytocompatibility (near 100 % viability). The results of the threedimensional (3D) cell culture also proved that the survival rate of the cells in the hybrid hydrogels doped with nHAP and dispersion were the highest. In vitro RT-qPCR experiments proved that after being cultured in hydrogel scaffolds doped with nHAP, bone mesenchymal stem cells (BMSCs) could express genes related to osteoblasts and chondrocytes. As a result, this hydrogel provides a general for developing alternative materials applicable for stem cells differentiation and even osteochondral tissue engineering.
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