期刊
CARBOHYDRATE POLYMERS
卷 266, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2021.118138
关键词
Gold nanoparticles; Fluorescent probes; Peptides; Urokinase plasminogen activator receptor; Imaging
资金
- Science and Engineering Research Board (SERB-ECR), New Delhi, India [ECR/2016/000075]
- Deanship of Scientific Research at King Saud University [RG-1435-007]
Targeting cell surface receptors for specific drug delivery in cancer, especially uPAR, which is overexpressed on many tumors, can be efficiently targeted and imaged using peptide-functionalized AuNPs.
Targeting cell surface receptors for specific drug delivery in cancer has garnered lot of attention. Urokinase plasminogen activator receptor (uPAR), a surface biomarker, is overexpressed on many tumours including breast, colorectal, prostate, and ovarian cancers. Binding of growth factor domain (GFD) of urokinase plasminogen activator (uPA) with uPAR lead to its close conformation, and allow somatomedin B domain (SMB) of vitronectin binding by allosteric modulation. In-silico docking of uPAR with GFD and SMB peptides was performed to identify potential binding affinity. Herein, we report fluorescently labeled peptide functionalized AuNPs with a mixed self-assembled monolayer of intercalating chitosan polymer for efficient targeting and imaging of uPARpositive cells. The biophysical characterization of nanoconjugates and uPAR-specific targeting was assessed by FACS, cell adhesion, and fluorescence imaging. AuNPs/chitosan/GFD+SMB peptides showed higher uptake as compared to AuNPs/chitosan/GFD, and AuNPs/chitosan/SMB that can be utilized as a tool for molecular targeting and imaging in metastasis.
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