Synthesis and characterization of glycol chitosan coated selenium nanoparticles acts synergistically to alleviate oxidative stress and increase ginsenoside content in Panax ginseng

The objective of the present study is synthesis of glycol chitosan coated selenium nanoparticles (GC-Se NPs) and evaluation of oxidative stress and ginsenoside accumulation in P. ginseng C. A. Meyer. We synthesized (Se NPs and GC-Se NPs) and characterized using various spectroscopic analyses. The highest concentration (20 mg L-1) of GC-Se NPs induced moderate ROS (O-2(center dot-) and H2O2) accumulation and upregulation of PgSOD and PgCAT showing good biocompatibility and less toxicity at the highest concentration. Furthermore, ginsenoside biosynthetic pathway genes (PgHMGR, PgSS, PgSE, PgDDS) also showed significant upregulation upon 20 mg L-1 GC-Se NPs treatment. At 20 mg L-1 GC-Se NPs treatment, ginsenoside accumulated upto 217.47 mg/mL and 169.86 mg/mL mainly due to the increased proportion of Rb1 and Re ginsenosides. Altogether, our results suggested that ecofriendly conjugation of GC with Se NPs could be used as a bio fortifier to enhance the ginsenoside profile and to increase the quality of ginseng roots.

Automated summary

The study successfully synthesized glycol chitosan coated selenium nanoparticles (GC-Se NPs) and found that treatment with 20 mg/L GC-Se NPs can induce oxidative stress and increase ginsenoside accumulation in Panax ginseng C. A. Meyer, leading to enhanced ginsenoside content and potential as a bio fortifier for improving ginseng root quality.