Preparation of chitosan biguanidine/PANI-containing self-healing semi-conductive waterborne scaffolds for bone tissue engineering

Electrically conducting self-healing scaffolds are known as a new series of intelligent biomaterial for regulating Human Adipose Mesenchymal Stem Cells biological behaviors, especially their differentiation to bone cells. Herein, we developed a novel hydrophilic semi-conductive chitosan derivative (CP) and loaded it into the selfhealing waterborne polyurethane structure, as a new osteogenic agent. The fabricated scaffolds exhibited excellent shape memory properties with shape fixity (> 97 %) and shape recovery ratio (> 98 %) with excellent self-healing value (> 93 %) at a temperature close to the body temperature. The results of MTT, cell attachment, alkaline phosphatase activity, and alizarin red staining analysis demonstrated that the CP-contained scaffolds promote proliferation of hADSCs and matrix mineralization. Also, by introducing the CP the gene expression level of COL-1, ALP, RUNX2, and OCN were significantly enhanced, in line with matrix mineralization. These multifunctional engineered constructs are promising biomaterials for repairing various bone defects.

Automated summary

Electrically conducting self-healing scaffolds loaded with a novel osteogenic agent CP were developed to regulate the biological behaviors of human Adipose Mesenchymal Stem Cells. The fabricated scaffolds exhibited excellent shape memory properties and high self-healing value, promoting cell proliferation and matrix mineralization. By enhancing gene expression levels related to bone formation, these engineered constructs show promise as biomaterials for repairing bone defects.