期刊
CANCER SCIENCE
卷 112, 期 10, 页码 4377-4392出版社
WILEY
DOI: 10.1111/cas.15063
关键词
allelic expression imbalance; DNA methylation; esophageal squamous cell carcinoma; imprinted gene; integrative analysis
类别
资金
- Conquering cancer through NEO-dimensional systems understandings [15H05908]
- Japan Agency for Medical Research and Development (AMED)
- Nanken-Kyoten, TMDU
- [16K14630]
The study revealed frequent variants and copy number amplifications in cancer-related genes among esophageal squamous cell carcinoma (ESCC) patients, with two mutational signatures observed in Japanese cases. Aberrant DNA methylation in imprinted genes and frequent nonsynonymous single-nucleotide variants in FAT family genes were also identified. The integrative genome-wide analyses showed altered gene regulation in ESCC.
Esophageal squamous cell carcinoma (ESCC) is a malignant disease. At present, the genomic profiles of ESCC are known to a considerable extent, and DNA methylation and gene expression profiles have been mainly used for the classification of ESCC subtypes, but integrative genomic, transcriptomic, and epigenomic analyses remain insufficient. Therefore, we performed integrative analyses using whole-exome sequencing, DNA methylation, and RNA sequencing (RNA-seq) analyses of Japanese patients with ESCC. In cancer-related genes, such as NOTCH family genes, RTK/PI3K pathway genes, and NFE2L2 pathway genes, variants and copy number amplification were detected frequently. Japanese ESCC cases were clustered into two mutational signatures: an APOBEC-associated signature and an age-related signature. In imprinted genes, DNA methylation was aberrant in gene promoter regions and correlated well with gene expression profiles. Nonsynonymous single-nucleotide variants and allelic expression imbalance were detected frequently in FAT family genes. Our integrative genome-wide analyses, including DNA methylation and allele-specific gene expression profiles, revealed altered gene regulation of imprinted genes and FAT family genes in ESCC.
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