期刊
CANCER LETTERS
卷 514, 期 -, 页码 79-89出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.canlet.2021.05.011
关键词
RHAMM; Pancreatic ductal adenocarcinoma; Pancreatic neuroendocrine tumor; Mouse models; p53
类别
资金
- NIH [R01CA204916-01A1]
- DoD [W81XWH-16-1-0619]
- Center for Translational Pathology at the Department of Pathology and Laboratory Medicine, Weill Cornell Medicine
- STARR [I12-0043]
Pancreatic cancer has the lowest survival rate and urgent need for better treatment; RHAMM is a potential therapeutic target for pancreatic cancer; HMMRAexon8-16 lacking centrosome targeting domain accelerates cancer progression.
Pancreatic cancer has the lowest survival rate out of all types of cancer. Pancreatic cancer patients are often diagnosed at advanced stages, hence an urgent need for a better therapeutic development of this devastating disease. Receptor for hyaluronan-mediated motility (RHAMM), not expressed in adult normal pancreas, has been suggested as a prognostic factor and a potential therapeutic target for pancreatic ductal adenocarcinoma (PDAC) and pancreatic neuroendocrine tumor (PNET). In this study, we initially sought to determine whether genetic deletion of RHAMM would slow down pancreatic cancer progression using Rhamm- /- mice. However, we found that Rhamm-/- mice expressed a truncated HMMRAexon8-16 protein at higher abundance levels than wild-type RHAMM. While HMMRAexon8-16 did not enable malignant progression of pancreatic intraepithelial neoplasia in p48-Cre; LSL-KRASG12D mice, it accelerated the formation of invasive PDAC and shortened the survival of p48Cre; LSL-KRASG12D mice with heterozygous p53 knockout. KrasG12D PDAC mice with homozygous p53 knockout mice died around 10 weeks, and the effect of HMMRAexon8-16 was not apparent in these short lifespan mice. In addition, HMMRAexon8-16 shortened the survival of PNET-bearing RIP-Tag mice, which had inactivated p53. In our analysis of TCGA dataset, pancreatic cancer patients with mutant TP53 or loss of one copy of TP53 had higher RHAMM expression, which, combined, predicted worse outcomes. Taken together, by collaborating with dysfunctional p53, high levels of HMMRAexon8-16 , which lacks the centrosome targeting domain and degrons for interaction with the Anaphase-Promoting Complex (APC), accelerated pancreatic cancer progression.
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