期刊
CANCER CELL
卷 39, 期 8, 页码 1150-+出版社
CELL PRESS
DOI: 10.1016/j.ccell.2021.05.005
关键词
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资金
- Ruth L. Kirschstein National Research Service [F30-DK120135]
- Blavatnik Family Fellowship in Biomedical Research [T32-HD083185]
- National Human Genome Research Institute [R00HG010152]
- National Cancer Institute [5P30CA023108-37, R01-CA168654]
- Howard Hughes Medical Institute
- Shipley Foundation Program for Innovation in Stem Cell Science
- Allen Discovery Center for Cell Lineage Tracing
This study introduces a new inducible CRISPR-Cas9-based lineage recorder, macsGESTALT, and reveals that the metastatic potential of cells peaks in rare, late-hybrid EMT states, which are relevant to clinical disease progression.
The underpinnings of cancer metastasis remain poorly understood, in part due to a lack of tools for probing their emergence at high resolution. Here we present macsGESTALT, an inducible CRISPR-Cas9-based lineage recorder with highly efficient single-cell capture of both transcriptional and phylogenetic information. Applying macsGESTALT to a mouse model of metastatic pancreatic cancer, we recover similar to 380,000 CRISPR target sites and reconstruct dissemination of similar to 28,000 single cells across multiple metastatic sites. We find that cells occupy a continuum of epithelial-to-mesenchymal transition (EMT) states. Metastatic potential peaks in rare, late-hybrid EMT states, which are aggressively selected from a predominately epithelial ancestral pool. The gene signatures of these late-hybrid EMT states are predictive of reduced survival in both human pancreatic and lung cancer patients, highlighting their relevance to clinical disease progression. Finally, we observe evidence for in vivo propagation of S100 family gene expression across clonally distinct metastatic subpopulations.
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