From bench to bedside: Single-cell analysis for cancer immunotherapy

Single-cell technologies are emerging as powerful tools for cancer research. These technologies characterize the molecular state of each cell within a tumor, enabling new exploration of tumor heterogeneity, microenvironment cell-type composition, and cell state transitions that affect therapeutic response, particularly in the context of immunotherapy. Analyzing clinical samples has great promise for precision medicine but is technically challenging. Successfully identifying predictors of response requires well-coordinated, multi-disciplinary teams to ensure adequate sample processing for high-quality data generation and computational analysis for data interpretation. Here, we review current approaches to sample processing and computational analysis regarding their application to translational cancer immunotherapy research.

Automated summary

Single-cell technologies are powerful tools for cancer research, providing insights into tumor heterogeneity and cell state transitions affecting therapeutic response. Analyzing clinical samples is promising for precision medicine but requires coordination among multi-disciplinary teams for data accuracy and interpretation.