期刊
BRITISH JOURNAL OF HAEMATOLOGY
卷 196, 期 2, 页码 374-379出版社
WILEY
DOI: 10.1111/bjh.17801
关键词
high-sensitivity flow cytometry; B-lymphoblastic leukaemia measurable residual disease; mimics and artefacts; multicolour flow cytometry measurable residual disease; normal in measurable residual disease
类别
资金
- National Cancer Grid, Tata Memorial Centre
The study identifies six consistently present low-level populations in 441 BMRD samples from 301 children, which immunophenotypically mimic abnormal B-ALL blasts. Understanding the presence and immunophenotype of these mimics is crucial for accurate interpretation in high-sensitivity MFC-BMRD analysis.
High-sensitivity multicolour flow cytometry (MFC)-based B-lymphoblastic leukaemia (B-ALL) measurable residual disease (BMRD) assay is increasingly being used in clinical practice. Herein, we describe six consistently present low-level populations immunophenotypically mimicking abnormal B-ALL blasts in 441 BMRD samples from 301 children. These included CD19(+)CD123(+) plasmacytoid dendritic cells differentiating from lymphoid precursors, CD10(+) transitional B cells with CD10(+)/CD38dim-to-negative/CD20bright/CD45bright phenotype, CD19(+) natural killer (NK) cells, CD73bright/CD10(+) mesenchymal stromal/stem cells, CD73bright/CD34(+) endothelial cells, and a CD34(+)CD38dim-to-negative/CD10(-)/CD20bright/CD45bright subset of mature B cells. We provide the proportions, comprehensive immunophenotype, and practical clues for proper identification of these low-level populations. Knowledge regarding the presence and immunophenotype of these mimics is essential for accurate interpretation in high-sensitivity MFC-BMRD analysis.
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