4.6 Article

Transcriptomic changes during stage progression of mycosis fungoides

期刊

BRITISH JOURNAL OF DERMATOLOGY
卷 186, 期 3, 页码 520-531

出版社

WILEY
DOI: 10.1111/bjd.20760

关键词

-

资金

  1. Canadian Dermatology Foundation [CDF RES0035718]
  2. University Hospital Foundation (University of Alberta, UofA)
  3. Bispebjerg Hospital (Copenhagen, Denmark)
  4. Danish Cancer Society (Kraeftens Bekaempelse) [R124-A7592 Rp12350]
  5. Department of Medicine, UofA
  6. Alberta Innovates Summer Research Studentship
  7. Canadian Institutes of Health Research (CIHR)
  8. Alberta Innovates
  9. UofA

向作者/读者索取更多资源

The study revealed that progression in Mycosis fungoides (MF) is associated with the activation of cell proliferation, survival, as well as the Th2/Th9 signaling pathway in malignant cells. There is evidence of cell migration between late-stage lesions, suggesting a possibility of cell percolation between different skin lesions.
Background Mycosis fungoides (MF) is the most common cutaneous T-cell lymphoma, which in the early patch/plaque stages runs an indolent course. However, similar to 25% of patients with MF develop skin tumours, a hallmark of progression to the advanced stage, which is associated with high mortality. The mechanisms involved in stage progression are poorly elucidated. Objectives We sought to address the hypothesis of MF cell trafficking between skin lesions by comparing transcriptomic profiles of skin samples in different clinical stages of MF. Methods We performed whole-transcriptome and whole-exome sequencing of malignant MF cells from skin biopsies obtained by laser-capture microdissection. We compared three types of MF lesions: early-stage plaques (ESP, n = 12) as well as plaques and tumours from patients in late-stage disease [late-stage plaques (LSP, n = 10) and tumours (TMR, n = 15)]. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were used to determine pathway changes specific for different lesions which were linked to the recurrent somatic mutations overrepresented in MF tumours. Results The key upregulated pathways during stage progression were those related to cell proliferation and survival (MEK/ERK, Akt-mTOR), T helper cell (Th)2/Th9 signalling [interleukin (IL)4, STAT3, STAT5, STAT6], meiomitosis (CT45A1, CT45A3, STAG3, GTSF1, REC8) and DNA repair (PARP1, MYCN, OGG1). Principal coordinate clustering of the transcriptome revealed extensive gene expression differences between early (ESP) and advanced-stage lesions (LSP and TMR). LSP and TMR showed remarkable similarities at the level of the transcriptome, which we interpreted as evidence of cell percolation between lesions via haematogenous self-seeding. Conclusions Stage progression in MF is associated with Th2/Th9 polarization of malignant cells, activation of proliferation, survival, as well as increased genomic instability. Global transcriptomic changes in multiple lesions may be caused by haematogenous cell percolation between discrete skin lesions.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.6
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据