期刊
BMC GENOMICS
卷 22, 期 1, 页码 -出版社
BMC
DOI: 10.1186/s12864-021-07731-x
关键词
Transcriptome; Spleen; Stress erythropoiesis; Immune response; Hypoxia
资金
- Chinese Academy of Sciences -People's Government of Qinghai Province on Sanjiangyuan National Park [LHZX-2020-01]
- Second Tibetan Plateau Scientific Expedition and Research Program (STEP) [2019QZKK0501]
- Qinghai Key R&D and Transformation Program [2019-SF-150]
- Science and Technology Department of Qinghai Province Major Project Sanjiangyaun National Park Animal Genome Program
The study found that exposure to hypoxia led to a significant increase in erythroid progenitor proliferation in mice spleen, accompanied by a decrease in overall gene expression. Stress erythropoiesis was regulated by three transcription factors, and the expression of genes related to immune response was downregulated.
BackgroundThe spleen is the largest secondary lymphoid organ and the main site where stress erythropoiesis occurs. It is known that hypoxia triggers the expansion of erythroid progenitors; however, its effects on splenic gene expression are still unclear. Here, we examined splenic global gene expression patterns by time-series RNA-seq after exposing mice to hypoxia for 0, 1, 3, 5, 7 and 13days.ResultsMorphological analysis showed that on the 3rd day there was a significant increase in the spleen index and in the proliferation of erythroid progenitors. RNA-sequencing analysis revealed that the overall expression of genes decreased with increased hypoxic exposure. Compared with the control group, 1380, 3430, 4396, 3026, and 1636 genes were differentially expressed on days 1, 3, 5, 7 and 13, respectively. Clustering analysis of the intersection of differentially expressed genes pointed to 739 genes, 628 of which were upregulated, and GO analysis revealed a significant enrichment for cell proliferation. Enriched GO terms of downregulated genes were associated with immune cell activation. Expression of Gata1, Tal1 and Klf1 was significantly altered during stress erythropoiesis. Furthermore, expression of genes involved in the immune response was inhibited, and NK cells decreased.ConclusionsThe spleen of mice conquer hypoxia exposure in two ways. Stress erythropoiesis regulated by three transcription factors and genes in immune response were downregulated. These findings expand our knowledge of splenic transcriptional changes during hypoxia.
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