期刊
BMC GASTROENTEROLOGY
卷 21, 期 1, 页码 -出版社
BMC
DOI: 10.1186/s12876-021-01722-8
关键词
Post-infectious irritable bowel syndrome; Gamma delta T cells; Subset; Function; Pathogenesis
资金
- Natural Science Foundation of China [81160057, 81860102]
- International Science and Technique Corporation Foundation of Hainan Province, China [KJHZ2013-14]
In patients with PI-IBS, the V delta 1 γδ T cells significantly increased in both peripheral blood and local colonic tissue, while V delta 2 γδ T cells remained unchanged. Furthermore, V delta 1 γδ T cells demonstrated remarkable proliferation, activation, and IL-17 production when cultured in vitro.
Background: gamma delta T cells play an important role in the mucosa inflammation and immunity-associated disorders. Our previous study reported that gamma delta T cells producing IL-17 were involved in the pathogenesis of post-infectious irritable bowel syndrome (PI-IBS). However, their subset characteristic profile in this kind of disease remains unclear. Thus the current study's aim is to investigate the functionally predominant subset and its role in PI-IBS. Methods: The total T cells were collected from the peripheral blood of patients with PI-IBS. The peripheral proportion of V delta 1 and V delta 2 subset was detected by FACS after stained with anti delta 1-PE and anti delta 2-APC. The local colonic proportion of this two subsets were measured under laser confocal fluorescence microscope. V delta 1 gamma delta T cells were enriched from the total peripheral T cells by minoantibody-immuno-microbeads (MACS) method and cultured, functionally evaluated by CCK-8 assay (proliferation), CD69/CD62L molecules expression assay (activation) and ELISA (IL-17 production) respectively. Results: 1. V delta 1 gamma delta T cells significantly increased while V delta 2 gamma delta T cells remained unchanged in both the peripheral blood and local colonic tissue from PI-IBS patients (p < 0.05). 2. When cultured in vitro, the V delta 1 gamma delta T cells remarkably proliferated, activated and produced IL-17 (p < 0.05). Conclusions: Our results suggest that V delta 1 gamma delta T cells was the predominant gamma delta T cells subset in both peripheral and intestinal tissue, and was the major IL-17 producing gamma delta T cells in PI-IBS.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据