期刊
EXPERIMENTAL GERONTOLOGY
卷 75, 期 -, 页码 1-7出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.exger.2015.11.021
关键词
Aging; Lung; Melatonin; Inflammation; Oxidative stress; Apoptosis
资金
- Mutua Madrilena
- Red Cooperativa de Envejecimiento y Fragilidad, Instituto de Salud Carlos III, Madrid (Spain) [RETICEF-RD12/0043/0032]
- Rodriguez Pascual Foundation
Aging is associated with an increase in oxidative stress and inflammation. The aging lung is particularly affected since it is continuously exposed to environmental oxidants while antioxidant machinery weakens with age. Melatonin, a free radical scavenger, counteracts inflammation and apoptosis in healthy cells from several tissues. Its effects on the aging lung are, however, not yet fully understood. This study aimed to investigate the effect of chronic administration of melatonin on the expression of inflammation markers (TNF-alpha, IL-1 beta, NF kappa B2, HO-1) and apoptosis parameters (BAD, BAX, AIF) in the lung tissue of male senescence-accelerated prone mice (SAMP8). In addition, RNAoxidative damage, as the formation of 8-hydroxyguanosine (8-OHG), was also evaluated. Young andoldanimals, aged 2 and 10 months respectively, were divided into 4 groups: untreated young, untreated old, old mice treated with 1 mg/kg/day melatonin, and old animals treated with 10 mg/kg/day melatonin. Untreated young and old male senescence accelerated resistant mice (SAMR1) were used as controls. After 30 days of treatment, animals were sacrificed. Lungs were collected and immediately frozen in liquid nitrogen. mRNA and protein expressions were measured by RT-PCR and Western blotting, respectively. Levels of 8-OHG were quantified by ELISA. Mean values were analyzed using ANOVA. Old nontreated SAMP8 animals showed increased (p < 0.05) mRNA and protein levels of TNF-alpha, IL-1 beta, NF kappa B2, and HO-1 compared to young mice and SAMR1 mice. Melatonin treatment with either dose reversed the aging-derived inflammation (p < 0.05). BAD, BAX and AIF expressions also rose with aging, the effect being counteracted with melatonin (p < 0.05). Aging also caused a significant elevation (p b 0.05) in SAMP8 8-OHG values. This increase was not observed in animals treated with melatonin (p < 0.05). In conclusion, melatonin treatment was able to modulate the inflammatory and apoptosis status of the aging lungs, exerting a protective effect on age-induced damage. (C) 2015 Elsevier Inc. All rights reserved.
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