4.6 Article

Drawing up a collaborative contract: Amino acid cross-feeding between interspecies bacterial pairs

期刊

BIOTECHNOLOGY AND BIOENGINEERING
卷 118, 期 8, 页码 3138-3149

出版社

WILEY
DOI: 10.1002/bit.27837

关键词

coculture; cross-feeding; microbial community; synthetic ecology

资金

  1. Division of Chemical, Bioengineering, Environmental, and Transport Systems [1403815]
  2. Division of Molecular and Cellular Biosciences [1055676]
  3. Direct For Biological Sciences
  4. Div Of Molecular and Cellular Bioscience [1055676] Funding Source: National Science Foundation
  5. Div Of Chem, Bioeng, Env, & Transp Sys
  6. Directorate For Engineering [1403815] Funding Source: National Science Foundation

向作者/读者索取更多资源

Synthetic microbial communities can benefit from cross-feeding interactions to promote growth and stability, as demonstrated by experiments on different culture conditions.
Synthetic microbial communities have the potential to enable new platforms for bioproduction of biofuels and biopharmaceuticals. However, using engineered communities is often assumed to be difficult because of anticipated challenges in establishing and controlling community composition. Cross-feeding between microbial auxotrophs has the potential to facilitate coculture growth and stability through a mutualistic ecological interaction. We assessed cross-feeding between 13 Escherichia coli amino acid auxotrophs paired with a leucine auxotroph of Bacillus megaterium. We developed a minimal medium capable of supporting the growth of both bacteria and used the media to study coculture growth of the 13 interspecies pairs of auxotrophs in batch and continuous culture, as well as on semi-solid media. In batch culture, 8 of 13 pairs of auxotrophs were observed to grow in coculture. We developed a new metric to quantify the impact of cross-feeding on coculture growth. Six pairs also showed long-term stability in continuous culture, where coculture growth at different dilution rates highlighted differences in cross-feeding amongst the pairs. Finally, we found that cross-feeding-dependent growth on semi-solid media is highly stringent and enables identification of the most efficient pairs. These results demonstrate that cross-feeding is a viable approach for controlling community composition within diverse synthetic communities.

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