期刊
BIOTECHNOLOGY ADVANCES
卷 54, 期 -, 页码 -出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.biotechadv.2021.107798
关键词
Mimic living cells; Enzymes; Enzyme compartmentalization; Sequential reactions; Polymersome; Layer-by-layer assembly
资金
- Villum Fonden [00028005]
- Polish National Agency for Academic Exchange [PPN/BEK/2020/1/00068]
Engineering microenvironments for sequential enzymatic reactions has attracted specific interest in improving the catalytic performance of enzymes. This review discusses current and potential methods of fabricating artificial cells for sequential enzymatic reactions, inspired by living cells' mechanisms and metabolic pathways. Layer-by-layer assembly of polyelectrolytes is highlighted as an important method for developing multi-compartments.
Engineering microenvironments for sequential enzymatic reactions has attracted specific interest within different fields of research as an effective strategy to improve the catalytic performance of enzymes. While in industry most enzymatic reactions occur in a single compartment carrier, living cells are however able to conduct multiple reactions simultaneously within confined sub-compartments, or organelles. Engineering multi-compartments with regulated environments and transformation properties enhances enzyme activity and stability and thus increases the overall yield of final products. In this review, we discuss current and potential methods to fabricate artificial cells for sequential enzymatic reactions, which are inspired by mechanisms and metabolic pathways developed by living cells. We aim to advance the understanding of living cell complexity and its compartmentalization and present solutions to mimic these processes in vitro. Particular attention has been given to layer-by-layer assembly of polyelectrolytes for developing multi-compartments. We hope this review paves the way for the next steps toward engineering of smart artificial multi-compartments with adoptive stimuli-responsive properties, mimicking living cells to improve catalytic properties and efficiency of the enzymes and enhance their stability.
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