Selective target protein detection using a decorated nanopore into a microfluidic device

Solid-state nanopores provide a powerful tool to electrically analyze nanoparticles and biomolecules at singlemolecule resolution. These biosensors need to have a controlled surface to provide information about the analyte. Specific detection remains limited due to nonspecific interactions between the molecules and the nanopore. Here, a polymer surface modification to passivate the membrane is performed. This functionalization improves nanopore stability and ionic conduction. Moreover, one can control the nanopore diameter and the specific interactions between protein and pore surface. The effect of ionic strength and pH are probed. Which enables control of the electroosmotic driving force and dynamics. Furthermore, a study of polymer chain structure and permeability in the pore are carried out. The nanopore chip is integrated into a microfluidic device to ease its handling. Finally, a discussion of an ionic conductance model through a permeable crown along the nanopore surface is elucidated. The proof of concept is demonstrated by the capture of free streptavidin by the biotins grafted into the nanopore. In the future, this approach could be used for virus diagnostic, nanoparticle or biomarker sensing.

Automated summary

Solid-state nanopores provide a powerful tool for single-molecule analysis of nanoparticles and biomolecules. Surface modification with polymers enhances stability and ionic conduction, allowing for control over nanopore diameter and specific interactions with proteins. Factors such as ionic strength and pH are investigated for their effects on the system, improving control over electroosmotic driving force and dynamics.