4.8 Article

Design and development of ferrocene-containing chitosan-cografted-branched polyethylenimine redox conjugates for monitoring free flap failure after reconstructive surgery

期刊

BIOSENSORS & BIOELECTRONICS
卷 186, 期 -, 页码 -

出版社

ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2021.113283

关键词

Ferrocene-containing redox polymers; Continuously metabolites monitoring; Free flap; Electrochemical biosensor

资金

  1. National Research Foundation of Singapore, Central Gap Fund [NRF2017NRF-CG001-003]

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The study developed an electrochemical biosensor for continuous monitoring of free flap failure caused by vascular occlusion. By attaching target enzymes to build the sensors, changes in metabolite levels could be detected within minutes.
We reported a proof-of-concept study of developing an electrochemical biosensor for prolonged continuous monitoring free flap failure caused by vascular occlusion after reconstructive surgery. Ferrocene (Fc)-containing Chitosan-cografted-Branched Polyethylenimine redox conjugates (CHIT-Fc-co-BPEI-Fc) were used as pHtuneable matrix to attach the target enzymes (glucose oxidase = GOD and lactate oxidase = LOD, respectively) to build up the corresponding GOD-sensor and LOD-sensor. The sensitivity of GOD-/LOD-sensor was found to be 2.89(+/- 0.06)mu A/mM and 2.95(+/- 0.19)mu A/mM with a limit of detection (LOD) of 0.047 mM and 0.172 mM, respectively. The sensor performance was further pre-clinically validated via rabbit study, which confirmed that the designed biosensors could electrochemically detect the changes of metabolite levels caused by flap failure within minutes. This sensor protocol is able to be fabricated as embedded biosensor for prolong continuous detection for clinical application/research.

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